Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Biotin
| Applications | ELISA: 0.5µg/ml - 1.0µg/ml. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Secondary Antibodies
Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Biotin
| Applications | ELISA: 500ng/ml. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Biotin
| Applications | ELISA: 125ng/ml as detecting antibody. |
| Reactivities | Rat |
| Conjugation | Biotin |
Rat IgD (heavy chain) mouse monoclonal antibody, clone MARD-3, Biotin
| Applications | ELISA: 500 ng/ml. |
| Reactivities | Rat |
| Conjugation | Biotin |
Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Biotin
| Applications | ELISA: 500 ng/ml. |
| Reactivities | Rat |
| Conjugation | Biotin |
Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, Biotin
| Applications | ELISA: 500ng/ml. |
| Reactivities | Rat |
| Conjugation | Biotin |
Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, Biotin
| Applications | ELISA: 500ng/ml. |
| Reactivities | Rat |
| Conjugation | Biotin |
Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Biotin
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/150,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions ELISA: 1/20,000-1/100,000. Immunohistochemistry: 1/1,000-1/5,000. Western blot: 1/2,000-1/10,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Human IgG (gamma chain specific) rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | Biotin |
Canine IgG (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
Canine IgA (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
Canine IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000. |
| Reactivities | Canine |
| Conjugation | Biotin |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin
| Applications | ELISA. Western Blot (1/5000). Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate. |
| Reactivities | Chicken |
| Conjugation | Biotin |
Human IgE goat polyclonal antibody, Biotin
| Applications | ELISA: 1/10,000-1/100,000. Western Blot: 1/10,000-1/100,000. |
| Reactivities | Human, Monkey |
| Conjugation | Biotin |
Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Biotin
| Applications | ELISA: 1/5,000-1/20,000. Immunofluorescence < / = 1 µg/10e6 cells. |
| Reactivities | Human |
| Conjugation | Biotin |
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