CNY 9630.00
5周
Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), FITC
| Applications | To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. General Recommended Dilutions: Histochemical Use: 1/10-1/50. ELISA: from 1/200 upwards. Western blot: from 1/400 upwards. |
| Reactivities | Human |
| Conjugation | FITC |





![Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n [B501-0500]) 1 Hour at 20°C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescien Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/609-302-002-human-igg-antibody-fluorescein-conjugated-1-db-4x3.jpg?browse)