Fluorescein Conjugated Mouse IgG (H&L)
| Applications | WB: User Optimized IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | FITC |
Secondary Antibodies
Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Assay Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-5,000. |
| Reactivities | Chicken |
| Conjugation | FITC |
Porcine IgA goat polyclonal antibody, FITC
| Applications | Flow Cytometry. Immunohistochemistry on Frozen Sections: 1/200-1/2000. |
| Reactivities | Porcine |
| Conjugation | FITC |
Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, FITC
| Applications | Flow Cytometry: 5 µg/ml; use 5 µl of the suggested working dilution to label 10e6 cells in 100µl. |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, FITC
| Applications | Flow Cytometry: Use 10 µl of 2.5 µg/ml diluted antibody to label 106 cells in 100 µl. |
| Reactivities | Mouse |
| Conjugation | FITC |
Canine IgA (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80, depending on the method used. |
| Reactivities | Canine |
| Conjugation | FITC |
Monkey IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. |
| Reactivities | Monkey |
| Conjugation | FITC |
Monkey IgG (H+L chain) goat polyclonal antibody, FITC
| Applications | To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80. |
| Reactivities | Monkey |
| Conjugation | FITC |
Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/20-1/80. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | FITC |
Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, FITC
| Applications | Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80. |
| Reactivities | Monkey |
| Conjugation | FITC |
Canine IgG (H+L chain) goat polyclonal antibody, FITC
| Applications | Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/120. |
| Reactivities | Canine |
| Conjugation | FITC |
Canine IgG (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgG isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/15 and 1/60, depending on the method used. |
| Reactivities | Canine |
| Conjugation | FITC |
Mouse IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80. |
| Reactivities | Mouse |
| Conjugation | FITC |
Canine IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. General Recommended Dilutions: are usually between 1/10 and 1/40, depending on the method used. |
| Reactivities | Canine |
| Conjugation | FITC |
Turkey IgG (H+L chain) goat polyclonal antibody, FITC
| Applications | Immunofluorescence: < / = 1 µg/10e6 cells. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry. |
| Reactivities | Turkey |
| Conjugation | FITC |
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