Secondary Antibodies

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Sheep IgG (H+L chain) Rabbit Polyclonal Antibody
AP33082FC-N

CNY 6730.00

5周

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.
Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC
Sheep IgG (H+L chain) Rabbit Polyclonal Antibody
AP33082AF-N

CNY 2825.00

5周

Sheep IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.
Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated
Sheep IgG (Fc specific) Rabbit Polyclonal Antibody
AP33081FC-N

CNY 7710.00

5周

Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC
Sheep IgG (Fc specific) Rabbit Polyclonal Antibody
AP33081AF-N

CNY 6580.00

5周

Sheep IgG (Fc specific) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.
Can be used As unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5.000.
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated
Sheep IgM Rabbit Polyclonal Antibody
SP2130P

CNY 3200.00

4周

Sheep IgM rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Sheep
Conjugation Unconjugated
Sheep IgM Rabbit Polyclonal Antibody
SP2130F

CNY 3675.00

4周

Sheep IgM rabbit polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2000.
Reactivities Sheep
Conjugation FITC
Sheep IgM Rabbit Polyclonal Antibody
SP2130HRP

CNY 3800.00

4周

Sheep IgM rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Sheep
Conjugation HRP
Sheep IgA Rabbit Polyclonal Antibody
SP2129P

CNY 3200.00

4周

Sheep IgA rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Sheep
Conjugation Unconjugated
Sheep IgG F(c) specific(adsorbed) Rabbit Polyclonal Antibody
R1381P

CNY 3994.00

4周

Sheep IgG F(c) specific(adsorbed) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity..
Reactivities Sheep
Conjugation Unconjugated
Sheep IgG F(ab)2 specific (adsorbed) Rabbit Polyclonal Antibody
R1383T

CNY 4144.00

4周

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation TRITC
Sheep IgG F(ab)2 specific (adsorbed) Rabbit Polyclonal Antibody
R1383TR

CNY 4819.00

4周

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation Texas Red
Sheep IgG F(ab)2 specific Rabbit Polyclonal Antibody
R1382HRP

CNY 3713.00

4周

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, HRP

Applications Suitable for Western Blot (1:1,000- 1:10,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (1:500- 1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 m g of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation HRP
Sheep IgG F(ab)2 specific Rabbit Polyclonal Antibody
R1382AP

CNY 4144.00

4周

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation AP
Sheep IgG F(ab)2 specific Rabbit Polyclonal Antibody
R1382B

CNY 3544.00

4周

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:160,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation Biotin
Sheep IgG F(c) specific (adsorbed) Rabbit Polyclonal Antibody
R1381T

CNY 4369.00

4周

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation TRITC