Human IgA rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100). Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Incubation Time: 30 minutes at room temperature. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |





![Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule (p/n 010-0102). Lane 2: Mouse IgG F(c) Fragment (p/n 010-0103). Lane 3: Mouse IgG F(ab) Fragment (p/n 010-0105). Lane 4: Mouse IgM Kappa (p/n 010-0107). Lane 5: Mouse Serum (p/n [D308-05]). Load: 50ng per lane. Block: (p/n MB-070) for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n 705-703-125) 1:40,000 for 30 min at RT . Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/610-1102-anti-mouse-igg-1-wb-4x3.jpg?browse)

![SDS PAGE Results of Goat Anti-Mouse IgG Antibody. Lane 1: Goat Anti-Mouse IgG Reduced [1.0µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500).
Lane 3: Goat Anti-Mouse IgG Non-Reduced [1.0µg]. 4-20% Gel, Coomassie Stained.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/210-1102-gt-a-mouseigg-1-sds-4x3.jpg?browse)

