Secondary Antibodies
Human IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgD (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in nonisotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/50 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/10,000. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgD (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgD in human serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/250 ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. Performance testing. Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Human |
| Conjugation | Biotin |
CNY 15460.00
5周
Human IgA (IgAc, A1, A2, Secretory) mouse monoclonal antibody, clone NI 69 and NI 184+NI 69-11+NI 512+NI 194, Ascites
| Applications | Can be used to identify the presence of IgA and secretory subclasses in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting. Recommended Working Dilutions: Histochemical Use: 1/100-1/500. ELISA: from 1/1000 Upwards. Western blotting: 1/2000-1/10000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 15460.00
5周
Human IgG, IgA, IgM, IgD, IgE mouse monoclonal antibody, clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307, Ascites
| Applications | Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting. Recommended Working Dilutions: Histochemical Use: 1/100-1/500. ELISA: from 1/1000 Upwards. Western blotting: 1/2000 Upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 3940.00
5周
Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, HRP
| Applications | ELISA: 0.1-0.5 µg/ml. Western blot. |
| Reactivities | Human |
| Conjugation | HRP |
CNY 9630.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), HRP
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/20 upwards. ELISA: form 1/50 upwards. Western blot: from 1/100 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgE goat polyclonal antibody, AP
| Applications | ELISA: 1/1000-1/10000. Western Blot: 1/1000-1/5000. |
| Reactivities | Human, Monkey |
| Conjugation | AP |
CNY 3861.00
4周
Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, HRP
| Applications | ELISA: 1/2,000-1/8,000. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Biotin
| Applications | ELISA: 1/5,000-1/10,000. Western blotting. Dot- and Slot-Immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
| Conjugation | Biotin |
Human IgE goat polyclonal antibody, FITC
| Applications | Flow Cytometry. |
| Reactivities | Human, Monkey |
| Conjugation | FITC |
CNY 3718.00
4周
Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, AP
| Applications | ELISA: 1/1,000-1/2,000. |
| Reactivities | Human |
| Conjugation | AP |
CNY 2750.00
5周
Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, Aff - Purified
| Applications | Immunocytochemistry. Western Blotting: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM. Flow cytometry: 1-4 μg/ml. ELISA: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments by ELISA. The antibody EM-07 does not crossreact with IgM. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgA rabbit polyclonal antibody, Aff - Purified
| Applications | Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200. |
| Reactivities | Human |
| Conjugation | Unconjugated |
