Antibodies

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
ELISA: 1/20-1/500.
Western Blot: 1/40-1/1000.
Histochemical Use: 1/5-1/20.
Reactivities Human
Conjugation HRP

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, HRP

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay.
Reactivities Human
Conjugation HRP

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), HRP, Purified

Applications ELISA: 1/4000-1/8000 (See also Ref.2-9).
Applications Reported in Literature:
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation HRP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP

Applications ELISA: 1/4,000-1/10,000.
Western blotting.
Dot- and Slot-immunoblotting.
Immunohistochemistry on Frozen Sections: 1/10-1/20.
Reactivities Human
Conjugation HRP

6X His Tag Antibody HRP Conjugated

Applications ELISA, IHC, WB

TRITC mouse monoclonal antibody, clone NI 308, HRP

Applications ELISA, IF, IHC, WB

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), HRP

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/100 upwards.
Western blot: from 1/250 upwards.
Reactivities Human
Conjugation HRP