Antibodies
Alb rabbit polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, R, WB |
| Reactivities | Mouse |
Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100- 1/2500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin
| Applications | ELISA: 1/1000 - 1/2000. Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Lyve1 rabbit polyclonal antibody, Biotin
| Applications | ELISA, FC, IHC, WB |
| Reactivities | Mouse, Rat |
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Isotypes
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- Chemokine signaling pathway (1)
- ECM-receptor interaction (1)
- Fc epsilon RI signaling pathway (1)
- Fc gamma R-mediated phagocytosis (1)
- Focal adhesion (1)
- GnRH signaling pathway (1)
- Neuroactive ligand-receptor interaction (1)
- Neurotrophin signaling pathway (1)
- Tight junction (1)
- Type II diabetes mellitus (1)
- Vascular smooth muscle contraction (1)
