Antibodies
Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Monkey IgG (gamma chain specific) goat polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting (Western Blot: 1/10,000-1/40,000), ELISA (1/700,000), Immunohistochemistry (1/1,000-1/2,500), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. |
| Reactivities | Monkey |
| Conjugation | Biotin |
Canine IgG (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
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- LDHA (5)
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Reactivities
- Human (105)
- Mouse (67)
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- Rabbit (45)
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- Rat (32)
- Escherichia coli (18)
- Monkey (16)
- Bakers Yeast (15)
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Applications
Antibody Hosts
Assay Types
Families
Pathways
- Chemokine signaling pathway (4)
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- Metabolic pathways (3)
- Citrate cycle (TCA cycle) (2)
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- Cytokine-cytokine receptor interaction (2)
- ECM-receptor interaction (2)
- Fc epsilon RI signaling pathway (2)
- Fc gamma R-mediated phagocytosis (2)
- Glyoxylate and dicarboxylate metabolism (2)
- GnRH signaling pathway (2)
- Neuroactive ligand-receptor interaction (2)
- Pyruvate metabolism (2)
- Systemic lupus erythematosus (2)
- Tight junction (2)
- Vascular smooth muscle contraction (2)
- Adipocytokine signaling pathway (1)
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- Butanoate metabolism (1)
- Calcium signaling pathway (1)
- Cytosolic DNA-sensing pathway (1)
- ErbB signaling pathway (1)
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- Insulin signaling pathway (1)
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- MAPK signaling pathway (1)
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- Neurotrophin signaling pathway (1)
- Nitrogen metabolism (1)
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- Pathways in cancer (1)
- Phosphatidylinositol signaling system (1)
- Porphyrin and chlorophyll metabolism (1)
- Prion diseases (1)
- Regulation of autophagy (1)
- Riboflavin metabolism (1)
- Taste transduction (1)
- Type II diabetes mellitus (1)
- VEGF signaling pathway (1)
- Vibrio cholerae infection (1)
- Wnt signaling pathway (1)
- aspartate and glutamate metabolism (1)
- mTOR signaling pathway (1)
