Bovine IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | Immunoblotting (1/2,000-1/10,000). ELISA (1/20,000-1/100,000). Immunohistochemistry (1/1,000-1/5,000). Immunomicroscopy. as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | Biotin |
