Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified
| Applications | Western blot. Recommended dilution: 1 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining. Immunocytochemistry. ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Purified
| Applications | ELISA (both capture and detection). Western Blot. Radioimmunoassay |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
| Conjugation | Unconjugated |
Human IgG2 (Hinge Region) mouse monoclonal antibody, clone 3C7, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
CNY 7710.00
5周
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified
| Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone 904, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425
| Applications | Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user. |
| Reactivities | Porcine |
| Conjugation | Unconjugated |
CNY 3113.00
4周
Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified
| Applications | Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells. ELISA. Immunohistochemistry on Frozen Sections. Western blotting. Dot- and Slot-Immunoblotting. Immunocytochemistry. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM (Mu chain specific) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended dilutions: ELISA: 1/10,000-1/45,000. Western blot: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Monkey IgA (Secretory component) goat polyclonal antibody, Azide Free
| Applications | Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Working dilutions: Histochemistry: 1/50 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/1000. |
| Reactivities | Chimpanzee, Monkey |
| Conjugation | Unconjugated |
Monkey IgG (Fab specific) rabbit polyclonal antibody, Azide Free
| Applications | Can be used for indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. |
| Reactivities | Monkey |
| Conjugation | Unconjugated |
