Antibodies

Chicken IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Conjugation Unconjugated

Canine IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/3000.
Reactivities Canine
Conjugation Unconjugated

Rabbit IgA goat polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Rabbit
Conjugation Unconjugated

Goat IgM rabbit polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Goat
Conjugation Unconjugated

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on paraffin sections.
Reactivities Mouse
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.)
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-2, Aff - Purified

Applications Immunoassay.
Immunohistochemistry on Frozen Sections.
The specificity of monoclonal antibody anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at a high level can induce cross-reactions with other IgG subclasses.
Reactivities Mouse
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone B572M, Purified

Applications ELISA.
Lateral flow assays.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone MK1A6, Purified

Applications ELISA assay development of total IgG.
Passive hemagglutination assays.
Histological staining of Acetone Fixed Frozen tissue sections.
Reactivities Human
Conjugation Unconjugated

Feline IgG mouse monoclonal antibody, clone GPB2-2B1, Purified

Applications ELISA: This antibody can bind to virus-bound immune IgG and can therefore detect anti-FeLV, FIV, FCV, FHV, FCoV/FIPV and FPV IgG activity in ELISA.
Western Blot: This antibody detects a band of approximately 50.0kDa on purified Feline IgG under reducing conditions.
Reactivities Feline
Conjugation Unconjugated

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Monkey IgA mouse monoclonal antibody, clone IgA5-3B, Purified

Applications ELISA.
Western Blot: Detects a band of approximately 55kDa under reducing conditions.
Reactivities Feline, Monkey
Conjugation Unconjugated

Mouse IgG rabbit polyclonal antibody, Serum

Reactivities Mouse
Conjugation Unconjugated

Human IgG rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Immunodiffusion.

For Ouchterlony:
Sample Size: 15 µl
Distance between wells: 5 mm
Test time: 4 days at 37°C
Antigens tested: Normal human serum, purified human IgG, normal mouse serum.
Results: Titre with NHS > 1:1000.
Reactivities Human
Conjugation Unconjugated

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone HIS40, Supernatant

Applications

Flow cytometry.
Immunofluorescence.
Immunohistochemistry on frozen tissue sections.

Reactivities Rat
Conjugation Unconjugated