Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
| Applications | WB: 1:1,000 - 1:5,000 IHC: 1:500 - 1:2,500 ELISA: 1:10,000 - 1:100,000 |
| Reactivities | Human |
| Conjugation | HRP |
Antibodies
Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western: 1/1,000-1/10,000 or dot blot), ELISA (1/10,000-1/50,000), immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | HRP |
F(ab')2 Human IgA (alpha chain) Antibody
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM mouse monoclonal antibody, clone M15/8, FITC
| Applications | Flow cytometry. |
| Reactivities | Human |
| Conjugation | FITC |
Human IgA + IgG + IgM goat polyclonal antibody, FITC
| Applications | Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl. |
| Reactivities | Human |
| Conjugation | FITC |
Human IgE mouse monoclonal antibody, clone IA2, HRP
| Applications | ELISA: 1/500 - 1/1000; This HRP conjugated antibody may be used as a detection reagent in a sandwich ELISA for IgE, utilising SM1814P as capture reagent. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgM mouse monoclonal antibody, clone R1/69, Supernatant
| Applications | Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections. Recommended Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 3410.00
5周
Kappa light chain, mouse anti human, clone κ -117 Biotin
| Applications | Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.25µg/ml (1:800) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil. |
| Reactivities | Human |
| Conjugation | Biotin |
Lambda light chain, mouse anti human, clone λ-1 Biotin
| Applications | Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.125µg/ml (1:1600) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil. |
| Reactivities | Human |
| Conjugation | Biotin |
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