Cy3 Conjugated Goat Anti-Mouse IgG
| Applications | IF/ICC: 1:50-100; IHC: 1:50-100 |
| Reactivities | Mouse IgG |
| Conjugation | Cy3 |
Antibodies
Guinea Pig IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/5000. |
| Reactivities | Guinea Pig |
| Conjugation | Biotin |
Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. |
| Reactivities | Monkey |
| Conjugation | FITC |
Bovine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western blot: 1/1,000-1/10,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | HRP |
Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Cy3 Conjugated Goat Anti-Rabbit IgG
| Applications | IF/ICC: 1:50-100; IHC: 1:50-100 |
| Reactivities | Rabbit IgG |
| Conjugation | Cy3 |
CNY 3113.00
4周
Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified
| Applications | Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells. ELISA. Immunohistochemistry on Frozen Sections. Western blotting. Dot- and Slot-Immunoblotting. Immunocytochemistry. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA: 1/100,000. Western blot: 1/500-1/3,000. Immunohistochemistry: 1/500-1/1,500. |
| Reactivities | Rat |
| Conjugation | HRP |
Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/3,000-1/25,000. Western blot: 1/1,000-1/4,000. Immunohistochemistry: 1/200-1/1,000. |
| Reactivities | Rabbit |
| Conjugation | AP |
CNY 7710.00
5周
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified
| Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone 904, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids). Immunocytochemistry. Immunohistochemistry. Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties. Dot blot. Western blot. General Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen. |
| Reactivities | Rabbit |
| Conjugation | HRP |
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Reactivities
- Mouse (494)
- Human (431)
- Rat (195)
- Rabbit (150)
- Goat (80)
- Chicken (73)
- Monkey (69)
- Sheep (65)
- Bovine (45)
- Guinea Pig (45)
- Hamster (39)
- Canine (34)
- Feline (30)
- Porcine (28)
- Horse (21)
- Dog (18)
- Pig (14)
- Duck (11)
- Equine (9)
- Donkey (7)
- Golden Syrian Hamster (7)
- Turkey (6)
- Chimpanzee (4)
- Mouse IgG (3)
- Human IgG (2)
- Reptiles (2)
- Goat IgG (1)
- Human Ig light chain (1)
- Human IgA (1)
- Human IgA1 (1)
- Human IgA2 (1)
- Human IgD (1)
- Human IgE (1)
- Human IgG1 (1)
- Human IgG3 (1)
- Human IgM (1)
- Human gamma heavy chain (1)
- Human kappa light chain (1)
- Human lambda light chain (1)
- Mammalian (1)
- Mouse Ig kappa light chain (1)
- Mouse IgG gamma (1)
- Mouse IgG1 (1)
- Mouse IgG2a kappa (1)
- Mouse IgG2b (1)
- Mouse IgM lambda light chain (1)
- Mouse IgM mu-chain (1)
- Rabbit IgG (1)
- Rabbit IgG Fab (1)
- Rabbit IgG Fc (1)
Applications
Antibody Hosts
Conjugates
- Unconjugated (700)
- FITC (453)
- HRP (447)
- Biotin (331)
- TRITC (243)
- Texas Red (206)
- AP (150)
- Alkaline Phosphatase (88)
- PE (60)
- Phycoerythrin (21)
- ATTO 425 (14)
- ATTO 532 (14)
- ATTO 550 (14)
- ATTO 594 (14)
- ATTO 647N (14)
- ATTO 655 (14)
- ATTO 488 (13)
- Agarose (12)
- Cy5 (6)
- APC (5)
- Cy3 (4)
- BGAL (1)
- BIMA (1)
- PE-Cy7 (1)
