Iba1 (149-161) goat polyclonal antibody, Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
| Applications | ELISA, IHC, WB |
| Reactivities | Hamster, Human, Monkey, Mouse, Porcine, Rat, Bovine, Equine, Goat, Rabbit, Pig |








![Immunoblot analysis of [AM02057PU-N]/AM02057PU-S specificty. Native and recombinant Bone proteins were separated by SDS-PAGE and immunoblotted with this BSP antibody Cat.-No [AM02057PU-N]/AM02057PU-S at 1/4000 dilution. This antibody did not detect BSP purified from Porcine Bone, nor Porcine Osteopontin (OPN) or SPARC/Osteonectin (ON). Gogan G et al. (2004) Connect Tissue Res 45 (1):60-71](https://cdn.origene.com/catalog/product/assets/images/antibody/acris/am02057pu-s-1-w.jpg?browse)
![ELISA standard curve showing measurement of Human BSP in a Sandwich Immunoassay using Cat.-No [AM20240PU-N]/[AM20240PU-S] as a Capture antibody and Cat.-No [AM02057PU-N]/AM02057PU-S as a Detection antibody. OD was determined at 450 nm. The shown standard curve is for demonstration purposes only and can be influenced by buffer, incubation conditions or dilution of the conjugate.](https://cdn.origene.com/catalog/product/assets/images/antibody/acris/am02057pu-s-1-e.jpg?browse)
![Immunohistochemistry image staining of BSP in Paraffin Section of Human Tissues. Sections were incubated with this BSP antibody Cat.-No [AM02057PU-N]/AM02057PU-S (1/2000) and detected using HRP conjugated secondary antibody and ABC Vectastain Elite Kit. DAB was used as the Chromogen. The sections were counterstained with Harris' Hematoxylin. Sections of B: Embryonic Tibia Sections of E: Primary breast Carcinoma Sections of G: Bone metastasis from the same patient were stained with this antibody. Inserted within B and E are Control Sections incubated with Mouse IgG. Scale bar = 20 µm. Gogan G et al. (2004) Connect Tissue Res 45 (1):60-71](https://cdn.origene.com/catalog/product/assets/images/antibody/acris/am02057pu-s-1-h.jpg?browse)
















