Expression Plasmids
HDGF (Myc-DDK-tagged)-Human hepatoma-derived growth factor (HDGF), transcript variant 1
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
MMP14 (Myc-DDK-tagged)-Human matrix metallopeptidase 14 (membrane-inserted) (MMP14)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
GNAQ (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), q polypeptide (GNAQ)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
AURKA (Myc-DDK-tagged)-Human aurora kinase A (AURKA), transcript variant 1
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
DUSP1 (Myc-DDK-tagged)-Human dual specificity phosphatase 1 (DUSP1)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
APOC3 (Myc-DDK-tagged)-Human apolipoprotein C-III (APOC3)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
UBE2D3 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
EGLN3 (Myc-DDK-tagged)-Human egl nine homolog 3 (C. elegans) (EGLN3)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
FKBP10 (Myc-DDK-tagged)-Human FK506 binding protein 10, 65 kDa (FKBP10)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
KLF5 (Myc-DDK-tagged)-Human Kruppel-like factor 5 (intestinal) (KLF5)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
IFNGR1 (Myc-DDK-tagged)-Human interferon gamma receptor 1 (IFNGR1)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
ORMDL3 (Myc-DDK-tagged)-Human ORM1-like 3 (S. cerevisiae) (ORMDL3)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
CTSD (Myc-DDK-tagged)-Human cathepsin D (CTSD)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
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Species
Target Symbol
- CD44 (10)
- AKT1 (9)
- MU OPIOID RECEPTOR (8)
- STAT3 (8)
- P63 (7)
- TRPA1 (7)
- UCP2 (7)
- ALDH1A1 (6)
- DNMT1 (6)
- FGFR2 (6)
- FOXA2 (6)
- FOXM1 (6)
- GATA2 (6)
- GPBAR1 (6)
- LDHA (6)
- NRP2 (6)
- P53 (6)
- PD-L1 (6)
- SOX6 (6)
- SPHK1 (6)
- TMEM16A (6)
- ABCG2 (5)
- AMYLOID PRECURSOR PROTEIN (5)
- BIRC5 (5)
- CERAMIDE SYNTHASE 2 (5)
- CONSTITUTIVE ANDROSTANE RECEPTOR (5)
- CREB1 (5)
- CXCR4 (5)
- DDIT3 (5)
- DNMT3A (5)
- DUSP6 (5)
- FYN (5)
- IRAK (5)
- IRF1 (5)
- KRAS (5)
- LAMP2 (5)
- MCL1 (5)
- MGMT (5)
- MITF (5)
- NLRP3 (5)
- NUMB (5)
- PLAUR (5)
- PRKACA (5)
- RUNX2 (5)
- S PROTEIN (5)
- SIRT1 (5)
- SIRT3 (5)
- SLC22A6 (5)
- SOD2 (5)
- SP1 (5)
- SQSTM1 (5)
- STAT1 (5)
- VEGFA (5)
- 5HT4 RECEPTOR (4)
- ABHD6 (4)
- ACSL1 (4)
- AGTR1A (4)
- ARF1 (4)
- B7H3 (4)
- BACE1 (4)
- BDNF (4)
- C6ORF134 (4)
- C9ORF72 (4)
- CAMK2A (4)
- CD63 (4)
- CHRNA3 (4)
- CTCF (4)
- CYP24A1 (4)
- DUSP5 (4)
- E2F1 (4)
- EGFR (4)
- EGR1 (4)
- ELAVL1 (4)
- ENDOTHELIN B RECEPTOR (4)
- FOXP3 (4)
- FSTL1 (4)
- GAPDH (4)
- GATA4 (4)
- GATA6 (4)
- GBA (4)
- GLP1R (4)
- GLUCOCORTICOID RECEPTOR (4)
- HES1 (4)
- ICAM1 (4)
- IGFBP1 (4)
- IGFBP7 (4)
- IRF5 (4)
- KLF4 (4)
- LAMIN A (4)
- LCK (4)
- LEGUMAIN (4)
- MDM2 (4)
- MLH1 (4)
- MMP14 (4)
- NEUROPILIN 1 (4)
- NFIB (4)
- NGF (4)
- NOR1 (4)
- NOX1 (4)
- NRF1 (4)
Vectors
Mutants
Families
- Druggable Genome (3342)
- Transmembrane (1691)
- Transcription Factors (889)
- Secreted Protein (468)
- ES Cell Differentiation/IPS (364)
- Protein Kinase (319)
- Stem cell - Pluripotency (191)
- GPCR (188)
- Protease (169)
- Adult stem cells (88)
- Embryonic stem cells (79)
- Phosphatase (66)
- Nuclear Hormone Receptor (63)
- Ion Channels: Other (58)
- Ion Channels: Potassium (55)
- Cancer stem cells (54)
- Induced pluripotent stem cells (51)
- Ion Channels: Transient receptor potential (29)
- P450 (28)
- Stem cell relevant signaling - DSL/Notch pathway (28)
- Stem cell relevant signaling - JAK/STAT signaling pathway (28)
- Ion Channels: Cys-loop Receptors (27)
- Stem cell relevant signaling - Wnt Signaling pathway (18)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (17)
- Ion Channels: Sodium (14)
- Ion Channels: Glutamate Receptors (11)
- Ion Channels: Calcium (6)
- Ion Channels: ATP Receptors (5)
Pathways
- Metabolic pathways (436)
- Pathways in cancer (364)
- MAPK signaling pathway (226)
- Neuroactive ligand-receptor interaction (173)
- Focal adhesion (166)
- Cytokine-cytokine receptor interaction (153)
- Regulation of actin cytoskeleton (147)
- Chemokine signaling pathway (144)
- Endocytosis (135)
- Jak-STAT signaling pathway (130)
- Neurotrophin signaling pathway (128)
- Prostate cancer (126)
- Wnt signaling pathway (120)
- Insulin signaling pathway (118)
- Calcium signaling pathway (115)
- T cell receptor signaling pathway (111)
- Alzheimer's disease (109)
- Colorectal cancer (106)
- Huntington's disease (105)
- ErbB signaling pathway (103)
- Axon guidance (99)
- Apoptosis (98)
- Toll-like receptor signaling pathway (98)
- Cell cycle (96)
- Chronic myeloid leukemia (95)
- Melanoma (94)
- Pancreatic cancer (91)
- Renal cell carcinoma (91)
- Leukocyte transendothelial migration (90)
- Natural killer cell mediated cytotoxicity (90)
- Cell adhesion molecules (CAMs) (86)
- Tight junction (86)
- Endometrial cancer (84)
- Small cell lung cancer (84)
- Melanogenesis (83)
- Glioma (82)
- Acute myeloid leukemia (81)
- Fc gamma R-mediated phagocytosis (80)
- TGF-beta signaling pathway (80)
- Ubiquitin mediated proteolysis (80)
- Adherens junction (79)
- VEGF signaling pathway (77)
- GnRH signaling pathway (76)
- Non-small cell lung cancer (76)
- Progesterone-mediated oocyte maturation (75)
- B cell receptor signaling pathway (74)
- p53 signaling pathway (74)
- Gap junction (71)
- Hematopoietic cell lineage (71)
- Adipocytokine signaling pathway (70)
- Fc epsilon RI signaling pathway (69)
- Vascular smooth muscle contraction (69)
- Lysosome (67)
- Bladder cancer (65)
- Oocyte meiosis (65)
- Purine metabolism (62)
- Parkinson's disease (61)
- RIG-I-like receptor signaling pathway (58)
- Long-term potentiation (56)
- mTOR signaling pathway (54)
- Epithelial cell signaling in Helicobacter pylori infection (53)
- Pathogenic Escherichia coli infection (53)
- Long-term depression (52)
- NOD-like receptor signaling pathway (52)
- Basal cell carcinoma (51)
- Type II diabetes mellitus (51)
- Spliceosome (50)
- Dilated cardiomyopathy (47)
- Thyroid cancer (46)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (45)
- Hypertrophic cardiomyopathy (HCM) (44)
- Amyotrophic lateral sclerosis (ALS) (43)
- ECM-receptor interaction (43)
- PPAR signaling pathway (43)
- Hedgehog signaling pathway (41)
- Antigen processing and presentation (40)
- Cytosolic DNA-sensing pathway (40)
- Oxidative phosphorylation (40)
- Metabolism of xenobiotics by cytochrome P450 (38)
- Notch signaling pathway (38)
- Prion diseases (38)
- Systemic lupus erythematosus (38)
- Glycolysis / Gluconeogenesis (37)
- Viral myocarditis (37)
- Glutathione metabolism (35)
- Maturity onset diabetes of the young (33)
- Vibrio cholerae infection (32)
- Drug metabolism - cytochrome P450 (31)
- Arachidonic acid metabolism (30)
- Dorso-ventral axis formation (29)
- Sphingolipid metabolism (29)
- Cardiac muscle contraction (28)
- Phosphatidylinositol signaling system (28)
- Pyruvate metabolism (28)
- Citrate cycle (TCA cycle) (27)
- Type I diabetes mellitus (27)
- Arginine and proline metabolism (26)
- Retinol metabolism (26)
- Autoimmune thyroid disease (25)
- Pyrimidine metabolism (25)
- ABC transporters (24)
- Complement and coagulation cascades (24)
- Cysteine and methionine metabolism (24)
- Lysine degradation (24)
- Allograft rejection (23)
- Glycerophospholipid metabolism (23)
- Graft-versus-host disease (23)
- Inositol phosphate metabolism (23)
- Tryptophan metabolism (23)
- Proteasome (22)
- Fatty acid metabolism (21)
- Base excision repair (20)
- DNA replication (19)
- Drug metabolism - other enzymes (19)
- Nicotinate and nicotinamide metabolism (18)
- Primary immunodeficiency (18)
- Starch and sucrose metabolism (18)
- Alanine (17)
- Glycerolipid metabolism (17)
- N-Glycan biosynthesis (17)
- Regulation of autophagy (17)
- Valine (17)
- aspartate and glutamate metabolism (17)
- Olfactory transduction (16)
- Tyrosine metabolism (16)
- Androgen and estrogen metabolism (15)
- Linoleic acid metabolism (15)
- RNA degradation (15)
- SNARE interactions in vesicular transport (15)
- Fructose and mannose metabolism (14)
- Glycine (14)
- Homologous recombination (14)
- Mismatch repair (14)
- Nucleotide excision repair (14)
- Ribosome (14)
- Taste transduction (14)
- leucine and isoleucine degradation (14)
- serine and threonine metabolism (14)
- Nitrogen metabolism (13)
- Propanoate metabolism (13)
- Galactose metabolism (12)
- Asthma (11)
- Butanoate metabolism (11)
- Pentose phosphate pathway (11)
- Aminoacyl-tRNA biosynthesis (10)
- Ether lipid metabolism (10)
- Phenylalanine metabolism (10)
- Renin-angiotensin system (10)
- Selenoamino acid metabolism (10)
- Amino sugar and nucleotide sugar metabolism (9)
- C21-Steroid hormone metabolism (9)
- Glycosphingolipid biosynthesis - lacto and neolacto series (9)
- Porphyrin and chlorophyll metabolism (9)
- Steroid biosynthesis (9)
- Biosynthesis of unsaturated fatty acids (8)
- Glycosaminoglycan degradation (8)
- Glyoxylate and dicarboxylate metabolism (8)
- Histidine metabolism (8)
- Heparan sulfate biosynthesis (7)
- Non-homologous end-joining (7)
- O-Glycan biosynthesis (7)
- Other glycan degradation (7)
- Pantothenate and CoA biosynthesis (7)
- Chondroitin sulfate biosynthesis (6)
- Glycosphingolipid biosynthesis - globo series (6)
- Keratan sulfate biosynthesis (6)
- Limonene and pinene degradation (6)
- One carbon pool by folate (6)
- Sulfur metabolism (6)
- alpha-Linolenic acid metabolism (6)
- leucine and isoleucine biosynthesis (6)
- Circadian rhythm - mammal (5)
- Folate biosynthesis (5)
- Primary bile acid biosynthesis (5)
- RNA polymerase (5)
- Synthesis and degradation of ketone bodies (5)
- beta-Alanine metabolism (5)
- Ascorbate and aldarate metabolism (4)
- D-Glutamine and D-glutamate metabolism (4)
- Glycosphingolipid biosynthesis - ganglio series (4)
- Methane metabolism (4)
- Pentose and glucuronate interconversions (4)
- Riboflavin metabolism (4)
- Terpenoid backbone biosynthesis (4)
- Caffeine metabolism (3)
- Phenylalanine (3)
- tyrosine and tryptophan biosynthesis (3)
- Basal transcription factors (2)
- Cyanoamino acid metabolism (2)
- Fatty acid elongation in mitochondria (2)
- Taurine and hypotaurine metabolism (2)
- Thiamine metabolism (2)
- Biotin metabolism (1)
- Fatty acid biosynthesis (1)
- Lipoic acid metabolism (1)
- Lysine biosynthesis (1)
- O-Mannosyl glycan biosynthesis (1)
- Protein export (1)
- Ubiquinone and other terpenoid-quinone biosynthesis (1)
- Vitamin B6 metabolism (1)


![HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY NBN (Cat# RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN(Cat# [TA801553]). Positive lysates [LY419300] (100ug) and [LC419300] (20ug) can be purchased separately from OriGene.](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta801553-1-w.jpg?browse)
![Western blot validation of overexpression lysate (Cat# [LY419300]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214682 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mg2722_d05.jpg?browse)

![HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY HDGF (Cat# RC204148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDGF (Cat# [TA808926])(1:500). Positive lysates [LY417948] (100ug) and [LC417948] (20ug) can be purchased separately from OriGene.](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta808926-1-w.jpg?browse)
![Western blot validation of overexpression lysate (Cat# [LY417948]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204148 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6283_c03.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY401554]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208917 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mg2550_d02.jpg?browse)
![Coomassie blue staining of purified MMP14 protein (Cat# [TP308917]). The protein was produced from HEK293T cells transfected with MMP14 cDNA clone (Cat# RC208917) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp308917.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY400763]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209199 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6126_d08.jpg?browse)
![Coomassie blue staining of purified GNAQ protein (Cat# [TP309199]). The protein was produced from HEK293T cells transfected with GNAQ cDNA clone (Cat# RC209199) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp309199.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY403678]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212018 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6119_g09.jpg?browse)
![Coomassie blue staining of purified AURKA protein (Cat# [TP312018]). The protein was produced from HEK293T cells transfected with AURKA cDNA clone (Cat# RC212018) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp312018.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY401403]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205220 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mg2339_d09.jpg?browse)
![HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY DUSP1 (Cat# RC205220, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP1 (Cat# [TA812680])(1:500). Positive lysates [LY401403] (100ug) and [LC401403] (20ug) can be purchased separately from OriGene.](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta812680-1-w.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY400010]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206566 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6016_a05.jpg?browse)
![Coomassie blue staining of purified APOC3 protein (Cat# [TP306566]). The protein was produced from HEK293T cells transfected with APOC3 cDNA clone (Cat# RC206566) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp306566.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY418758]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207371 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6023_f01.jpg?browse)
![Coomassie blue staining of purified UBE2D3 protein (Cat# [TP307371]). The protein was produced from HEK293T cells transfected with UBE2D3 cDNA clone (Cat# RC207371) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp307371.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY411796]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210319 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6267_e08.jpg?browse)
![Coomassie blue staining of purified EGLN3 protein (Cat# [TP310319]). The protein was produced from HEK293T cells transfected with EGLN3 cDNA clone (Cat# RC210319) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp310319.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY400087]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209259 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6126_h08.jpg?browse)
![Coomassie blue staining of purified LEP protein (Cat# [TP309259]). The protein was produced from HEK293T cells transfected with LEP cDNA clone (Cat# RC209259) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp309259.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY411871]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203676 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6245_a09.jpg?browse)
![Coomassie blue staining of purified FKBP10 protein (Cat# [TP303676]). The protein was produced from HEK293T cells transfected with FKBP10 cDNA clone (Cat# RC203676) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp303676.jpg?browse)

![HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY KLF5 (Cat# RC202438, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF5(Cat# [TA811868]). Positive lysates [LY419775] (100ug) and [LC419775] (20ug) can be purchased separately from OriGene.](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta811868-1-w.jpg?browse)
![Western blot validation of overexpression lysate (Cat# [LY419775]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202438 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mg3554_c01.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY400147]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202761 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mg2527_a07.jpg?browse)
![Coomassie blue staining of purified IFNGR1 protein (Cat# [TP302761]). The protein was produced from HEK293T cells transfected with IFNGR1 cDNA clone (Cat# RC202761) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp302761.jpg?browse)

![Western blot validation of overexpression lysate (Cat# [LY408336]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202279 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6359_g12.jpg?browse)
![Coomassie blue staining of purified ORMDL3 protein (Cat# [TP302279]). The protein was produced from HEK293T cells transfected with ORMDL3 cDNA clone (Cat# RC202279) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp302279.jpg?browse)

![HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY CTSD (Cat# RC200719, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTSD(Cat# [TA590104]). Positive lysates [LY400712] (100ug) and [LC400712] (20ug) can be purchased separately from OriGene.](https://cdn.origene.com/catalog/product/assets/images/antibody/116/ta590104-1-w.jpg?browse)
![Western blot validation of overexpression lysate (Cat# [LY400712]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200719 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/lysate/mk6052_g04.jpg?browse)