Collagen type I human protein, 0.5 mg
| Protein Source | Placenta |
Native Proteins
Mouse IgE mouse protein, 0.5 mg
| Protein Source | Cell culture |
Hemoglobin human protein, 2 g
| Protein Source | Erythrocytes |
Plasminogen / PLG human protein, 1 mg
| Protein Source | Plasma |
Collagen type IV human protein, 0.5 mg
| Protein Source | Placenta |
Trypsin-1 / PRSS1 human protein, 0.1 mg
| Protein Source | Pancreas |
Cytokeratin 18 bovine protein, 0.25 mg
| Protein Source | Liver |
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Species
Target Symbol
Families
- Druggable Genome (2)
- Secreted Protein (2)
- 10 mM Tris-HCl (1)
- 2 mM dithiothreitol (1)
-
ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting. (1) - ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays. (1)
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For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting. (1) - Protease (1)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (1) - Specific methodologies have not been tested using this product. (1)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (1)
-
e.g. Dulbeccos PBS).
See References 2 and 3 for more details. (1) -
pH 7.4).
For Immunization purposes (1) - the solution can be further dialyzed against PBS (phosphate buffered saline (1)
