Proteins

Proteins

Over-expression Lysates

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant Proteins

Native Proteins

Mutant CytoSections

Cell Line Lysates

Human

Mouse

SARS-CoV-2

Rat

Cynomolgus

Escherichia coli

Blomia tropicalis

Bovine

Dermatophagoides pteronyssinus

Orf Virus

Staphylococcus aureus

Streptococcus Pyogenes

Hepatitis B Virus

Chicken

Dermatophagoides farinae

Rabbit

Rhesus

Streptococcus sp.

Alternaria alternata

Betula pendula

Chlorocebus aethiops

Drosophila melanogaster

E.coli

Enterobacteria phage T4

Escherichia phage T7

HIV

Human herpesvirus

Human papillomavirus

Humon

Humulus japonicus

Lachnospiraceae bacterium ND2006

Lepidoglyphus destructor

Leptotrichia wadei

Macaca fascicularis

Mucor hiemalis

Mus musculus

Proteobacteria

SIV

Saccharomyces cerevisiae

Serratia marcescens

Staphylococcus aureus subsp.

Streptococcus

Streptomyces avidinii

Streptomyces plicatus

Tobacco etch virus

Vaccinia virus

viral

VEGFA

ALPHA 1 ANTITRYPSIN

NCALD

NDRG2

S PROTEIN

IRF5

RIBONUCLEASE INHIBITOR

FGFR1

HMGA1

ISOCITRATE DEHYDROGENASE

PQBP1

PRKAR1B

TPM1

MTH1

MYOSIN

ALKALINE PHOSPHATASE

ALPHA SYNUCLEIN

B3GALT5

CPNE1

FHL1

MBNL1

MORTALITY FACTOR 4 LIKE 2

PCBP2

UBE2D3

14-3-3 ZETA

ASPARTATE BETA HYDROXYLASE

FGF1

HNF 4 ALPHA

HNRNP F

IL6

M-CSF

MYELIN BASIC PROTEIN

NDUF3

P38

S100 CALCIUM BINDING PROTEIN A13

ANNEXIN A2

AURORA A

CD36

FGF2

FHL2

HER2

IDH2

MAX

PGF

APOLIPOPROTEIN E

B RAF

CARDIAC TROPONIN T

CD10

COASY

DGKA

ESTROGEN RELATED RECEPTOR GAMMA

FGL1

HNRNPD

HOMER2

ICAM2

IL4

LMO2

MBNL3

MPST

PEROXIREDOXIN 1

RAB27A

RNF14

TMEM169

UBE2I

YPEL5

ZFYVE27

AKT1

ALDOLASE

C2ORF88

C4BPB

CATHEPSIN B

CD33

CSF2

DOUBLECORTIN

EGFR

FCGRT

GIPC

GM CSF RECEPTOR ALPHA

GMPR2

IKK GAMMA

JNK1

LIF

OSBPL9

P53

PAX2

PPAR GAMMA

QK1

SEPTIN6

SIRP ALPHA

SPART

SULT1A1

SUPT5H

TNF ALPHA

TROPOMYOSIN 3

UBE2C

WDR79

ACRV1

BAIAP2

BETA CATENIN

BREAST CANCER SUPPRESSOR CANDIDATE 1

HEK293T

E. coli

HEK293

Sf9

Insect

CHO

Baculovirus

Pichia pastoris

Mouse

Other

Pichia

S. cerevisiae

Sf9 insect

H. vulgare

Human

Yeast

C-Myc/DDK

C-MYC/DDK

N-His

N-GST and C-His

C-Human Fc

C-His

C-DDK

C-6×His

N-GST and C-HIS

Tag Free

C-6His

C-DDK/His

His-Tag

Myc-DDK

C-Mouse Fc

N-His-ABP (Albumin-Binding Protein)

N-Human Fc

C-HIS

His-tag

C-Mouse Fc and 6×His

C-Fc

C-8His

C-hFc

N-His-Trx

N-6His

C-Human Fc and 6×His

N-GST, C-His

hIgG-His-Tag

N-HIS

C- 6×His

N-6×His

Tag-free

C-Avi&6His

C-10His

N-GST

hIgG-Tag

N-His PDCD1(Pro21-Val170)

N-His/DDK

N-Mouse Fc and C-6×His

C-6×His tag and C-Human Fc

C-mouse Fc

N-8His

N-Avi

N-His&C-His

N-His-GST

C-Fc/His

C-hFc&His

His-tagged, myc-tagged

N-DDK

C-3×Flag Tag

C-6xHis

C-6xHis/AVI

C-7×His

C-DDK-His

C-HUMAN

N-8His&N-FLAG

N-Fc-FLAG

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

C-AVI-PLUS

C-Avi-His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FC/6xHis

C-FLAG-Fc

C-Fc Human

C-Fc Mouse

C-HIS/C

C-HIS/DDK

C-His/AVIplus

C-His/DDK

C-Human Fc, Avi

C-Human Fc-Avi

C-hIgG1 Fc

C-hIgG4P Fc

C-mFc

C-terminal DDK

C-terminal His

DDK-tag

His tag

N-10*His

N-6His-FLAG

N-AVI

N-Avi&6His

N-DDK/His

N-FLAG

N-FUSION

N-Flag&C-His

N-GFP

N-GST(CKM) and N-His(CKB)

N-GST/C-His

N-HIS and C-AVIPLUS

N-PROTEIN

N-T7

N-TRX

N-TRXA

N-Trx-His

N-hFc

N-mFc

N-terminal His-tag

N-terminal murine Fc Tag

No

Tag free

PE

FITC

HRP

Yes

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

P450

GPCR

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

ELISA

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

SDS-PAGE

Ion Channels: Potassium

Bioactivity

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

Ion Channels: Cys-loop Receptors

e.g. Dulbecco's PBS).

Flow Cyt

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Functional Assays.

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

Metabolic pathways

Pathways in cancer

MAPK signaling pathway

Cytokine-cytokine receptor interaction

Focal adhesion

Regulation of actin cytoskeleton

Endocytosis

Chemokine signaling pathway

Neurotrophin signaling pathway

Jak-STAT signaling pathway

Insulin signaling pathway

Purine metabolism

Natural killer cell mediated cytotoxicity

T cell receptor signaling pathway

Toll-like receptor signaling pathway

Alzheimer's disease

Ubiquitin mediated proteolysis

Hematopoietic cell lineage

Wnt signaling pathway

Cell adhesion molecules (CAMs)

Leukocyte transendothelial migration

Cell cycle

Axon guidance

ErbB signaling pathway

Pancreatic cancer

Huntington's disease

Tight junction

Progesterone-mediated oocyte maturation

Prostate cancer

Apoptosis

GnRH signaling pathway

Fc epsilon RI signaling pathway

Colorectal cancer

Calcium signaling pathway

Adherens junction

Oocyte meiosis

Fc gamma R-mediated phagocytosis

Melanoma

Renal cell carcinoma

Chronic myeloid leukemia

Hypertrophic cardiomyopathy (HCM)

Spliceosome

Dilated cardiomyopathy

VEGF signaling pathway

Pyrimidine metabolism

B cell receptor signaling pathway

Lysosome

Small cell lung cancer

TGF-beta signaling pathway

NOD-like receptor signaling pathway

Glioma

RIG-I-like receptor signaling pathway

Adipocytokine signaling pathway

Systemic lupus erythematosus

Epithelial cell signaling in Helicobacter pylori infection

Parkinson's disease

Gap junction

Vascular smooth muscle contraction

PPAR signaling pathway

Bladder cancer

Melanogenesis

Acute myeloid leukemia

Glycolysis / Gluconeogenesis

Endometrial cancer

Pathogenic Escherichia coli infection

Non-small cell lung cancer

Complement and coagulation cascades

Phosphatidylinositol signaling system

Oxidative phosphorylation

Long-term potentiation

Cytosolic DNA-sensing pathway

Antigen processing and presentation

p53 signaling pathway

Long-term depression

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

mTOR signaling pathway

Amyotrophic lateral sclerosis (ALS)

Drug metabolism - cytochrome P450

Arginine and proline metabolism

Metabolism of xenobiotics by cytochrome P450

ECM-receptor interaction

Viral myocarditis

Cardiac muscle contraction

Inositol phosphate metabolism

Neuroactive ligand-receptor interaction

Thyroid cancer

Type II diabetes mellitus

Glycerophospholipid metabolism

Type I diabetes mellitus

Glutathione metabolism

Amino sugar and nucleotide sugar metabolism

Autoimmune thyroid disease

Retinol metabolism

Vibrio cholerae infection

Valine

Tyrosine metabolism

Starch and sucrose metabolism

Drug metabolism - other enzymes

Proteasome

leucine and isoleucine degradation

Alanine

Allograft rejection

Graft-versus-host disease

aspartate and glutamate metabolism

Tryptophan metabolism

Pyruvate metabolism

Fructose and mannose metabolism

RNA degradation

Prion diseases

Ribosome

Cysteine and methionine metabolism

Arachidonic acid metabolism

Fatty acid metabolism

Androgen and estrogen metabolism

Glycerolipid metabolism

Nucleotide excision repair

Hedgehog signaling pathway

Porphyrin and chlorophyll metabolism

Citrate cycle (TCA cycle)

Butanoate metabolism

Pentose phosphate pathway

Lysine degradation

DNA replication

Histidine metabolism

Base excision repair

Regulation of autophagy

Galactose metabolism

Notch signaling pathway

Olfactory transduction

Primary immunodeficiency

Propanoate metabolism

Nitrogen metabolism

Sphingolipid metabolism

Aminoacyl-tRNA biosynthesis

Basal cell carcinoma

beta-Alanine metabolism

Maturity onset diabetes of the young

Asthma

Linoleic acid metabolism

SNARE interactions in vesicular transport

Selenoamino acid metabolism

Ether lipid metabolism

Nicotinate and nicotinamide metabolism

Dorso-ventral axis formation

Glycine

serine and threonine metabolism

Mismatch repair

RNA polymerase

Phenylalanine metabolism

Glyoxylate and dicarboxylate metabolism

Ascorbate and aldarate metabolism

Pentose and glucuronate interconversions

Sulfur metabolism

Other glycan degradation

Riboflavin metabolism

Homologous recombination

Pantothenate and CoA biosynthesis

Renin-angiotensin system

Glycosaminoglycan degradation

Basal transcription factors

One carbon pool by folate

Taste transduction

alpha-Linolenic acid metabolism

Glycosphingolipid biosynthesis - lacto and neolacto series

Terpenoid backbone biosynthesis

Primary bile acid biosynthesis

C21-Steroid hormone metabolism

N-Glycan biosynthesis

O-Glycan biosynthesis

Limonene and pinene degradation

Folate biosynthesis

Glycosphingolipid biosynthesis - globo series

Thiamine metabolism

Biosynthesis of unsaturated fatty acids

Circadian rhythm - mammal

Heparan sulfate biosynthesis

Non-homologous end-joining

ABC transporters

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Steroid biosynthesis

Keratan sulfate biosynthesis

Synthesis and degradation of ketone bodies

Chondroitin sulfate biosynthesis

Glycosphingolipid biosynthesis - ganglio series

leucine and isoleucine biosynthesis

Methane metabolism

Taurine and hypotaurine metabolism

Caffeine metabolism

Fatty acid elongation in mitochondria

Phenylalanine

Protein export

Ubiquinone and other terpenoid-quinone biosynthesis

tyrosine and tryptophan biosynthesis

Fatty acid biosynthesis

Lysine biosynthesis

Vitamin B6 metabolism

D-Glutamine and D-glutamate metabolism

Cyanoamino acid metabolism

Biotin metabolism

Lipoic acid metabolism

Human

Human 293

E. coli

HEK293

293F

Insect

Expi293

P.Pichia

Pichia pastoris

1

2

3

Yes

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