Donor vector with AAVS1 homologous arms, C-terminal mGFP tag, Blasticidin resistance, ready for transgene cloning
| Function | AAVS1 donor vector |
Donor vector with AAVS1 homologous arms, C-terminal mGFP tag, Blasticidin resistance, ready for transgene cloning
| Function | AAVS1 donor vector |
pCMV6-AN-FC, mammalian vector with N-terminal FC tag, 10ug
| N-Tag | FC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Cell Selection | Neomycin |
pCMV6-AN-mYFP, mammalian vector with N-terminal mYFP, 10ug
| N-Tag | mYFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Cell Selection | Neomycin |
pCMV6-AN-YFP, mammalian vector with N-terminal tYFP tag, 10ug
| N-Tag | TurboYFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Cell Selection | Neomycin |
pCMV6-AN-Myc-DDK-AC-His, mammalian expression vector with N-terminal Myc-DDK tags and C-terminal His tag, 10 ug
| C-Tag | His |
| N-Tag | Myc-DDK |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Cell Selection | Neomycin |
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 D10A expression) (10 µg)
| Function | Cas9 Nickase |
pCMV6-Entry vector containing tGFP reporter gene
| C-Tag | Myc-DDK |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Cell Selection | Neomycin |
pCMV6-AN-His-HA, mammalian vector with N-terminal HA-His tag, 10ug
| N-Tag | His-HA |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Cell Selection | Neomycin |
Cas9 expression vector in AAVS1 donor vector, puromycin selection (10 ug)
| Function | Cas9 only vectors |
HuSH shRNA GFP Retroviral Cloning Vector with blasticidin resistance (pGFP-B-RS Vector) 5 ug
| Viral Packaging | Retroviral |
pLenti-Cas9-P2A-tGFP, Cas9 in lenti vector with tGFP after P2A (10 ug)
| Function | Cas9 only vectors |
pT7-Cas9-Nickase vector (Cas9 D10A) (10 µg)
| Function | Cas9 Nickase |
pLenti-Cas9 vector, Cas9 in lenti vector (10 ug)
| Function | Cas9 only vectors |
pAAV-AC-GFP, AAV vector with C-terminal tGFP tag and EF1a promoter
Vector kit to insert Cas9 at AAVS1 locus (puro selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-Puro donor (cat# GE100037), and a scramble gRNA control (cat# GE100003)
| Function | CRISPR/AAVS1 kit |