pCas-Guide-CRISPRa-Scramble, scramble gRNA control in GE100055 vector (no selection marker/reporter)
Function | CRISPRa scramble control vector |
pCas-Guide-CRISPRa-Scramble, scramble gRNA control in GE100055 vector (no selection marker/reporter)
Function | CRISPRa scramble control vector |
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 H840A expression) (10 µg)
Function | Cas9 Nickase |
pAAV-AC-GFP, AAV vector with C-terminal tGFP tag and EF1a promoter
Catalytically inactive Cas9 (D10A/H840A) vector for gRNA cloning (10 µg)
Function | Cas9 Nickase |
Cas9 expression vector in AAVS1 donor vector, blasticidin selection (10ug)
Function | Cas9 only vectors |
pCas-Guide-CRISPRa vector, All-in-one Vector for CRISPR/Cas9 Gene activation with dCas9-VP64 and gRNA cloning site.
Function | All-in-one CRISPRa SAM vector |
AAVS1 donor vector with AAVS1 homologous arms, tRFP under EF1a promoter, and Blasticidin selection (10 ug)
Function | AAVS1 donor vector |
HuSH shRNA Retroviral Cloning Vector with blasticidin resistance (pB-RS Vector), 5 ug
Viral Packaging | Retroviral |
pCas-Guide-Puro-CRISPRa-Scramble, scramble gRNA control in GE100081 vector (with Puro selection)
pLenti-C-Luciferase-P2A-Puro, Lenti vector with C-terminal firefly Luciferase tag and P2A-Puro, 10 ug
C-Tag | Luciferase |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-hSyn-C-mGFP-P2A-Puro, Lenti vector with Human synapsin I promoter, C-terminal mGFP tag and P2A-Puro, 10ug
C-Tag | mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-E/hSyn-C-Myc-DDK-P2A-Puro, Lenti vector with CMV enhancer, Human synapsin I promoter, C-terminal Myc-DDK tag and P2A-Puro, 10 ug
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-Cas9-mGFP-P2A-Puro control plasmid containing the expression cassette for Cas9-mGFP fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
pLenti-C-Myc-DDK-P2A-Puro-mGFP, Lenti vector with C-terminal Myc-DDK tag, P2A-Puro selection marker and mGFP reporter gene
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
HuSH shRNA RFP Lenti Cloning Vector (pRFP-C-shLenti Vector), 5 ug
Viral Packaging | Lentiviral |