CNY 9630.00
5周
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP
| Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. General Recommended Dilutions: ELISA: 1/20-1/500. Western Blot: 1/40-1/1000. Histochemical Use: 1/5-1/20. |
| Reactivities | Human |
| Conjugation | HRP |
Monkey IgA (Secretory component) goat polyclonal antibody, HRP
| Applications | Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working dilutions: Histochemical and cytochemical 1/150 - 1/500. ELISA and comparable non-precipitating antibody-binding assays 1/200 -1/4000. |
| Reactivities | Chimpanzee, Monkey |
| Conjugation | HRP |
Monkey IgG (Fc specific) goat polyclonal antibody, HRP
| Applications | Dot blot. Immunoblotting. ELISA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. This antibody can be used: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histo- and Cytochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000. |
| Reactivities | Monkey |
| Conjugation | HRP |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
| Reactivities | Mouse |
| Conjugation | HRP |
Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000. |
| Reactivities | Monkey |
| Conjugation | HRP |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids). Immunocytochemistry. Immunohistochemistry. Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties. Dot blot. Western blot. General Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen. |
| Reactivities | Rabbit |
| Conjugation | HRP |
Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, HRP
| Applications | This antibody can be used in Enzyme Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: Histochemical and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,0000. |
| Reactivities | Guinea Pig |
| Conjugation | HRP |
CNY 9630.00
5周
Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), HRP
| Applications | To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/10-1/100. ELISA: from 1/100 upwards. Western blot: from 1/250 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
Rat IgG2ab (subclass specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000 - 1/10,000. |
| Reactivities | Rat |
| Conjugation | HRP |
Rat IgG2b (subclass specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in Rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000. |
| Reactivities | Rat |
| Conjugation | HRP |
Mouse IgG3 (subclass specific) goat polyclonal antibody, HRP
| Applications | This antibody can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates To demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in Mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: Histochemical/Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/7,000 depending on the method used. Working dilutions schould be stored at +4°C, not refrozen, and preferably used the same day. |
| Reactivities | Mouse |
| Conjugation | HRP |
Rabbit IgA (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/50 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Rabbit |
| Conjugation | HRP |
Rabbit IgM (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000. |
| Reactivities | Rabbit |
| Conjugation | HRP |
Rat IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Reactivities | Rat |
| Conjugation | HRP |
Mouse IgE (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Mouse |
| Conjugation | HRP |
