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viral

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ZFYVE27

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HNF 4 ALPHA

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FHL2

GM CSF RECEPTOR ALPHA

ICAM2

MAX

NEK6

PPAR GAMMA

RAB27A

ACRV1

CARDIAC TROPONIN T

CPNE1

HNRNPD

HOMER2

JNK1

MPST

RGS3

SSH3BP1

TAU

YPEL5

ALDOLASE

C2ORF88

C4BPB

CASPASE-7

COASY

FGL1

GBA

GIPC

GMPR2

IKK GAMMA

LMO2

MEIS2

QK1

SEPTIN6

SPART

THYN1

TROPOMYOSIN 3

ARSA

CD36

IL32

MBNL3

MORTALITY FACTOR 4 LIKE 2

P53

PAX8

AKT1

APC11

ARF1

CAMK2D

CD32B

DYSTROPHIN

FXYD3

WB

IHC

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Baculovirus

Pichia pastoris

Mouse

Other

Pichia

S. cerevisiae

Sf9 insect

H. vulgare

Human

Yeast

C-Myc/DDK

C-MYC/DDK

N-His

N-GST and C-His

C-Human Fc

C-His

C-DDK

C-6×His

N-GST and C-HIS

Tag Free

C-6His

C-HIS

C-DDK/His

Myc-DDK

His-tag

C-Mouse Fc

N-His-ABP (Albumin-Binding Protein)

N-Human Fc

His-Tag

C-Mouse Fc and 6×His

C-Fc

C-8His

C-hFc

N-His-Trx

N-6His

C-Human Fc and 6×His

N-GST, C-His

hIgG-His-Tag

N-HIS

C- 6×His

N-6×His

Tag-free

C-Avi&6His

C-10His

N-GST

hIgG-Tag

N-His PDCD1(Pro21-Val170)

N-His/DDK

N-Mouse Fc and C-6×His

C-6×His tag and C-Human Fc

C-mouse Fc

N-8His

N-Avi

N-His&C-His

N-His-GST

C-Fc/His

C-hFc&His

His-tagged, myc-tagged

N-DDK

C-3×Flag Tag

C-6xHis

C-6xHis/AVI

C-7×His

C-DDK-His

C-HUMAN

C-Myc-DDK

N-8His&N-FLAG

N-Fc-FLAG

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

C-AVI-PLUS

C-Avi-His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FC/6xHis

C-FLAG-Fc

C-Fc Human

C-Fc Mouse

C-HIS/C

C-HIS/DDK

C-His/AVIplus

C-His/DDK

C-Human Fc, Avi

C-Human Fc-Avi

C-hIgG1 Fc

C-hIgG4P Fc

C-mFc

C-terminal DDK

C-terminal His

DDK-tag

His tag

N-10*His

N-6His-FLAG

N-AVI

N-Avi&6His

N-DDK/His

N-FLAG

N-FUSION

N-Flag&C-His

N-GFP

N-GST(CKM) and N-His(CKB)

N-GST/C-His

N-HIS and C-AVIPLUS

N-PROTEIN

N-T7

N-TRX

N-TRXA

N-Trx-His

N-hFc

N-mFc

N-terminal His-tag

N-terminal murine Fc Tag

No

Tag free

PE

FITC

Glucose Oxidase

HRP

Yes

MVPro

CytoSections

SDS PAGE

WB

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Ion Channels: Other

GPCR

Adult stem cells

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Potassium

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

ELISA

SDS-PAGE

Ion Channels: Glutamate Receptors

Ion Channels: Transient receptor potential

Ion Channels: ATP Receptors

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Ion Channels: Sodium

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

Ion Channels: Cyclic nucleotide gated

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Ion Channels: Calcium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Metabolic pathways

Pathways in cancer

MAPK signaling pathway

Cytokine-cytokine receptor interaction

Regulation of actin cytoskeleton

Focal adhesion

Endocytosis

Chemokine signaling pathway

Neurotrophin signaling pathway

Purine metabolism

Wnt signaling pathway

Jak-STAT signaling pathway

Insulin signaling pathway

Alzheimer's disease

Ubiquitin mediated proteolysis

Huntington's disease

Natural killer cell mediated cytotoxicity

Neuroactive ligand-receptor interaction

Calcium signaling pathway

Axon guidance

T cell receptor signaling pathway

Cell cycle

Tight junction

Toll-like receptor signaling pathway

Leukocyte transendothelial migration

Cell adhesion molecules (CAMs)

Progesterone-mediated oocyte maturation

ErbB signaling pathway

Hematopoietic cell lineage

Oocyte meiosis

Lysosome

Prostate cancer

GnRH signaling pathway

Apoptosis

Pancreatic cancer

Spliceosome

Fc gamma R-mediated phagocytosis

Colorectal cancer

Adherens junction

Systemic lupus erythematosus

Dilated cardiomyopathy

Pyrimidine metabolism

Hypertrophic cardiomyopathy (HCM)

Fc epsilon RI signaling pathway

Melanogenesis

VEGF signaling pathway

Melanoma

Parkinson's disease

Small cell lung cancer

Vascular smooth muscle contraction

Chronic myeloid leukemia

B cell receptor signaling pathway

Renal cell carcinoma

TGF-beta signaling pathway

Oxidative phosphorylation

Glioma

Antigen processing and presentation

Adipocytokine signaling pathway

Gap junction

PPAR signaling pathway

RIG-I-like receptor signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

NOD-like receptor signaling pathway

Acute myeloid leukemia

Olfactory transduction

Complement and coagulation cascades

Glycolysis / Gluconeogenesis

Phosphatidylinositol signaling system

p53 signaling pathway

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Long-term potentiation

Pathogenic Escherichia coli infection

Non-small cell lung cancer

Endometrial cancer

Long-term depression

Cardiac muscle contraction

Drug metabolism - cytochrome P450

Viral myocarditis

Cytosolic DNA-sensing pathway

ECM-receptor interaction

Metabolism of xenobiotics by cytochrome P450

Amyotrophic lateral sclerosis (ALS)

Glycerophospholipid metabolism

Arginine and proline metabolism

Bladder cancer

Ribosome

mTOR signaling pathway

Vibrio cholerae infection

Inositol phosphate metabolism

Valine

Thyroid cancer

Retinol metabolism

Amino sugar and nucleotide sugar metabolism

Glutathione metabolism

Drug metabolism - other enzymes

Type II diabetes mellitus

leucine and isoleucine degradation

RNA degradation

Starch and sucrose metabolism

Autoimmune thyroid disease

Proteasome

Tyrosine metabolism

Hedgehog signaling pathway

Arachidonic acid metabolism

Type I diabetes mellitus

Pyruvate metabolism

Fatty acid metabolism

Glycerolipid metabolism

Tryptophan metabolism

Alanine

aspartate and glutamate metabolism

Nucleotide excision repair

Fructose and mannose metabolism

Basal cell carcinoma

Cysteine and methionine metabolism

Graft-versus-host disease

Prion diseases

Notch signaling pathway

Lysine degradation

Androgen and estrogen metabolism

Allograft rejection

Butanoate metabolism

Base excision repair

Porphyrin and chlorophyll metabolism

Sphingolipid metabolism

Primary immunodeficiency

Pentose phosphate pathway

Citrate cycle (TCA cycle)

SNARE interactions in vesicular transport

DNA replication

Propanoate metabolism

Histidine metabolism

Aminoacyl-tRNA biosynthesis

Regulation of autophagy

Galactose metabolism

Nitrogen metabolism

Ether lipid metabolism

Taste transduction

Glycine

serine and threonine metabolism

Selenoamino acid metabolism

Linoleic acid metabolism

beta-Alanine metabolism

Maturity onset diabetes of the young

N-Glycan biosynthesis

Nicotinate and nicotinamide metabolism

Dorso-ventral axis formation

Glycosphingolipid biosynthesis - lacto and neolacto series

Asthma

RNA polymerase

Basal transcription factors

Homologous recombination

Mismatch repair

Phenylalanine metabolism

Sulfur metabolism

Glycosaminoglycan degradation

O-Glycan biosynthesis

Pantothenate and CoA biosynthesis

Pentose and glucuronate interconversions

Riboflavin metabolism

Ascorbate and aldarate metabolism

Other glycan degradation

Primary bile acid biosynthesis

Glyoxylate and dicarboxylate metabolism

Renin-angiotensin system

One carbon pool by folate

Terpenoid backbone biosynthesis

alpha-Linolenic acid metabolism

Biosynthesis of unsaturated fatty acids

Heparan sulfate biosynthesis

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Glycosphingolipid biosynthesis - globo series

C21-Steroid hormone metabolism

Limonene and pinene degradation

Keratan sulfate biosynthesis

Folate biosynthesis

Chondroitin sulfate biosynthesis

Circadian rhythm - mammal

Non-homologous end-joining

Steroid biosynthesis

leucine and isoleucine biosynthesis

ABC transporters

Thiamine metabolism

Glycosphingolipid biosynthesis - ganglio series

Taurine and hypotaurine metabolism

Caffeine metabolism

Synthesis and degradation of ketone bodies

Fatty acid elongation in mitochondria

Protein export

Cyanoamino acid metabolism

Ubiquinone and other terpenoid-quinone biosynthesis

Methane metabolism

Fatty acid biosynthesis

Phenylalanine

tyrosine and tryptophan biosynthesis

Lysine biosynthesis

Vitamin B6 metabolism

D-Glutamine and D-glutamate metabolism

Lipoic acid metabolism

Biotin metabolism

O-Mannosyl glycan biosynthesis

D-Arginine and D-ornithine metabolism

Human

Human 293

E. coli

Plasma

Spinal Cord

Brain

Liver

HEK293

Placenta

293F

Erythrocytes

Gizzard

Insect

Neutrophils

Stomach

Carcinoma

Cell culture

Egg white

Expi293

Leukocytes

P.Pichia

Pancreas

Pichia pastoris

Pituitary

S. avidinii

Serum

Synthetic

Urine

1

2

3

Yes

In Stock