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viral

ALPHA 1 ANTITRYPSIN

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RIBONUCLEASE INHIBITOR

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UBE2D3

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CONSTITUTIVE ANDROSTANE RECEPTOR

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S100 CALCIUM BINDING PROTEIN A13

ZFYVE27

ALPHA SYNUCLEIN

CD59

FHL1

ICAM2

P38

SULT1A1

UBE2I

MAX

ACRV1

CPNE1

FGL1

OSBPL9

HNF 4 ALPHA

PAX2

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ARSA

CAMK2B

DGKA

GM CSF RECEPTOR ALPHA

MORTALITY FACTOR 4 LIKE 2

NDUF3

ABLIM2

ASPARTATE BETA HYDROXYLASE

DOUBLECORTIN

DYSTROBREVIN ALPHA

FHL2

IL1RA

KALLIKREIN 11

NEK6

PPAR GAMMA

RAB27A

CARDIAC TROPONIN T

CD36

HNRNPD

HOMER2

JNK1

MPST

RGS3

SSH3BP1

TAU

TRKB

YPEL5

ALDOLASE

C2ORF88

C4BPB

CASPASE-7

COASY

GBA

GIPC

GMPR2

IKK GAMMA

LMO2

MEIS2

QK1

SEPTIN6

SPART

THYN1

GLUTAMINE SYNTHETASE

IL32

MBNL3

P53

PAX8

AKT1

APC11

ARF1

WB

IHC

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Baculovirus

Pichia

Pichia pastoris

Mouse

Other

S. cerevisiae

Sf9 insect

H. vulgare

Human

Yeast

C-Myc/DDK

C-MYC/DDK

N-His

C-His

Tag Free

N-GST and C-His

C-Fc

C-Human Fc

C-6His

C-DDK

C-6×His

N-GST and C-HIS

C-HIS

C-DDK/His

N-GST

Myc-DDK

His-tag

C-Mouse Fc

C-Fc&His

N-His-ABP (Albumin-Binding Protein)

N-Human Fc

C-mFc

His-Tag

N-His&C-His

C-6His-Avi

N-6His

C-Mouse Fc and 6×His

C-8His

N-T7&C-His

C-hFc

N-Trx&His

N-His-Trx

C-Fc-Avi

C-Human Fc and 6×His

C-Avi-6His

N-Fc

N-GST, C-His

hIgG-His-Tag

N-HIS

N-His&SUMO

C- 6×His

C-10His

N-6×His

Tag-free

C-Avi&6His

C-6xHis

hIgG-Tag

N-Avi

N-His PDCD1(Pro21-Val170)

N-His/DDK

N-Mouse Fc and C-6×His

C-6×His tag and C-Human Fc

C-mouse Fc

N-8His

N-GST&His

N-His&T7

N-His-GST

N-Trx

C-Fc/His

C-Flag

C-hFc&His

His-tagged, myc-tagged

N-DDK

N-mFc

C-3×Flag Tag

C-6xHis/AVI

C-7×His

C-DDK-His

C-FC

C-Fc-Flag&C-Fc-6His

C-HUMAN

C-Myc-DDK

N-6His-Avi

N-6His-Flag

N-8His&N-FLAG

N-FC

N-Fc-FLAG

N-His&Flag

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

C-AVI

C-AVI&His

C-AVI-PLUS

C-Avi

C-Avi-His

C-DDK/10xHis

C-DDK/6xHis

C-FC-6HIS&C-FC-Flag

C-FC/6xHis

C-FLAG-Fc

C-Fc Human

C-Fc Mouse

C-Flag,C-6His

C-GST

C-HIS/C

C-HIS/DDK

C-His&AVI

C-His&HA&AVI

C-His/AVIplus

C-His/DDK

C-Human Fc, Avi

C-Human Fc-Avi

C-hIgG1 Fc

C-hIgG2 Fc

C-hIgG4 Fc

C-hIgG4P Fc

C-mFC

C-mFc&His

C-mIgG2AFc

C-mIgG2a Fc

C-mIgG2aFc

C-terminal DDK

C-terminal His

DDK-tag

His tag

N-10*His

N-10His

N-10His-Flag

N-6His-FLAG

N-8His-Flag

N-AVI

N-Avi&6His

N-DDK/His

N-FLAG

N-FUSION

N-Flag&C-His

N-Flag&His

N-GFP

N-GST(CKM) and N-His(CKB)

N-GST/C-His

N-HIS and C-AVIPLUS

N-His&MBP

N-PROTEIN

N-T7

N-TRX

N-TRXA

N-Trx-His

N-hFc

N-terminal His-tag

N-terminal murine Fc Tag

No

Tag free

PE

FITC

Glucose Oxidase

HRP

Yes

MVPro

CytoSections

SDS PAGE

WB

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Ion Channels: Other

Adult stem cells

GPCR

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Potassium

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

ELISA

SDS-PAGE

Ion Channels: Glutamate Receptors

Ion Channels: Transient receptor potential

Ion Channels: ATP Receptors

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Ion Channels: Sodium

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

Ion Channels: Cyclic nucleotide gated

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Ion Channels: Calcium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Metabolic pathways

Pathways in cancer

MAPK signaling pathway

Cytokine-cytokine receptor interaction

Regulation of actin cytoskeleton

Focal adhesion

Endocytosis

Chemokine signaling pathway

Neurotrophin signaling pathway

Jak-STAT signaling pathway

Purine metabolism

Natural killer cell mediated cytotoxicity

Ubiquitin mediated proteolysis

Wnt signaling pathway

Alzheimer's disease

Insulin signaling pathway

Huntington's disease

T cell receptor signaling pathway

Cell adhesion molecules (CAMs)

Neuroactive ligand-receptor interaction

Calcium signaling pathway

Axon guidance

Hematopoietic cell lineage

Cell cycle

Toll-like receptor signaling pathway

Leukocyte transendothelial migration

Tight junction

ErbB signaling pathway

Progesterone-mediated oocyte maturation

Oocyte meiosis

Lysosome

Prostate cancer

Apoptosis

GnRH signaling pathway

Pancreatic cancer

Spliceosome

Adherens junction

Colorectal cancer

Fc gamma R-mediated phagocytosis

Melanoma

Systemic lupus erythematosus

Fc epsilon RI signaling pathway

Pyrimidine metabolism

Dilated cardiomyopathy

Hypertrophic cardiomyopathy (HCM)

Parkinson's disease

TGF-beta signaling pathway

Melanogenesis

B cell receptor signaling pathway

Chronic myeloid leukemia

Small cell lung cancer

VEGF signaling pathway

Vascular smooth muscle contraction

Renal cell carcinoma

Antigen processing and presentation

Glioma

Oxidative phosphorylation

RIG-I-like receptor signaling pathway

PPAR signaling pathway

Adipocytokine signaling pathway

Gap junction

NOD-like receptor signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Complement and coagulation cascades

Glycolysis / Gluconeogenesis

Acute myeloid leukemia

p53 signaling pathway

Olfactory transduction

Phosphatidylinositol signaling system

Long-term potentiation

Pathogenic Escherichia coli infection

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Endometrial cancer

Non-small cell lung cancer

Long-term depression

Viral myocarditis

Cytosolic DNA-sensing pathway

ECM-receptor interaction

Cardiac muscle contraction

Bladder cancer

Amyotrophic lateral sclerosis (ALS)

Arginine and proline metabolism

Metabolism of xenobiotics by cytochrome P450

Drug metabolism - cytochrome P450

Glycerophospholipid metabolism

mTOR signaling pathway

Ribosome

Inositol phosphate metabolism

Vibrio cholerae infection

Amino sugar and nucleotide sugar metabolism

Valine

Autoimmune thyroid disease

Glutathione metabolism

Drug metabolism - other enzymes

Thyroid cancer

Type II diabetes mellitus

Type I diabetes mellitus

Retinol metabolism

RNA degradation

leucine and isoleucine degradation

Starch and sucrose metabolism

Tyrosine metabolism

Pyruvate metabolism

Proteasome

Hedgehog signaling pathway

Arachidonic acid metabolism

Fructose and mannose metabolism

Fatty acid metabolism

Glycerolipid metabolism

Tryptophan metabolism

Graft-versus-host disease

Allograft rejection

Alanine

aspartate and glutamate metabolism

Basal cell carcinoma

Cysteine and methionine metabolism

Nucleotide excision repair

Prion diseases

Primary immunodeficiency

Androgen and estrogen metabolism

Porphyrin and chlorophyll metabolism

Notch signaling pathway

Lysine degradation

Base excision repair

Sphingolipid metabolism

Butanoate metabolism

Regulation of autophagy

Pentose phosphate pathway

Nitrogen metabolism

Citrate cycle (TCA cycle)

SNARE interactions in vesicular transport

Propanoate metabolism

Galactose metabolism

DNA replication

Aminoacyl-tRNA biosynthesis

Histidine metabolism

Ether lipid metabolism

Glycine

serine and threonine metabolism

Asthma

Taste transduction

Selenoamino acid metabolism

N-Glycan biosynthesis

Nicotinate and nicotinamide metabolism

beta-Alanine metabolism

Dorso-ventral axis formation

Linoleic acid metabolism

Glycosphingolipid biosynthesis - lacto and neolacto series

Maturity onset diabetes of the young

RNA polymerase

Sulfur metabolism

Basal transcription factors

Glycosaminoglycan degradation

Phenylalanine metabolism

Homologous recombination

Mismatch repair

Pantothenate and CoA biosynthesis

O-Glycan biosynthesis

Pentose and glucuronate interconversions

Renin-angiotensin system

Riboflavin metabolism

Other glycan degradation

Glyoxylate and dicarboxylate metabolism

Ascorbate and aldarate metabolism

Primary bile acid biosynthesis

Terpenoid backbone biosynthesis

One carbon pool by folate

alpha-Linolenic acid metabolism

Heparan sulfate biosynthesis

Biosynthesis of unsaturated fatty acids

Glycosphingolipid biosynthesis - globo series

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Keratan sulfate biosynthesis

C21-Steroid hormone metabolism

Limonene and pinene degradation

Folate biosynthesis

Chondroitin sulfate biosynthesis

Circadian rhythm - mammal

Steroid biosynthesis

Non-homologous end-joining

ABC transporters

leucine and isoleucine biosynthesis

Thiamine metabolism

Taurine and hypotaurine metabolism

Glycosphingolipid biosynthesis - ganglio series

Synthesis and degradation of ketone bodies

Caffeine metabolism

Fatty acid elongation in mitochondria

Methane metabolism

Ubiquinone and other terpenoid-quinone biosynthesis

Protein export

Cyanoamino acid metabolism

Fatty acid biosynthesis

Phenylalanine

tyrosine and tryptophan biosynthesis

Lysine biosynthesis

Vitamin B6 metabolism

D-Glutamine and D-glutamate metabolism

Lipoic acid metabolism

Biotin metabolism

O-Mannosyl glycan biosynthesis

D-Arginine and D-ornithine metabolism

Human

E. coli

Human 293

Plasma

Spinal Cord

P.Pichia

Brain

Liver

Baculovirus

HEK293

Placenta

293F

Erythrocytes

Gizzard

Insect

Neutrophils

Stomach

CHO Stable

Carcinoma

Cell culture

Egg white

Expi293

Leukocytes

Mammalian

Pancreas

Pichia pastoris

Pituitary

S. avidinii

Serum

Synthetic

Urine

1

2

3

Yes

In Stock