Vector kit for targeted transgene insertion into AAVS1 locus (EF1a, puro), including GE100023, GE100046 and GE100003. 2 vials of sequencing primers, 5' (EF51), 3' (XL39), 100 picomoles each, lyophilized
Function | CRISPR/AAVS1 kit |
Vector kit for targeted transgene insertion into AAVS1 locus (EF1a, puro), including GE100023, GE100046 and GE100003. 2 vials of sequencing primers, 5' (EF51), 3' (XL39), 100 picomoles each, lyophilized
Function | CRISPR/AAVS1 kit |
pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro, Lenti vector with EF1a promoter, N-terminal HA tag, C-terminal Myc-DDK tag and IRES-Puro, 10 ug
C-Tag | Myc-DDK |
N-Tag | HA |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-C-HA-IRES-BSD, Lenti vector with C-terminal HA tag and IRES-BSD, 10 ug
C-Tag | HA |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Blasticidin |
pLenti-C-Myc-DDK-P2A-tGFP, Lenti vector with C-terminal Myc-DDK tag and tGFP after P2A peptide, 10ug
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | tGFP |
pCMV6-XL5-DDK-His, mammalian vector with C-terminal DDK-His tag (No mammalian selection marker), 10ug
C-Tag | DDK-His |
E. coli Selection | Ampicillin (100 ug/mL) |
pCMV6-AN-mKate, mammalian vector with N-terminal mKate, 10ug
N-Tag | mKate |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |
pLenti-N-tGFP, Lenti Vector with N-terminal tGFP tag, 10ug
N-Tag | tGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Vector kit for targeted transgene insertion into ROSA26 locus in mice (puro), including GE100050, GE100051 and GE100003
Function | CRISPR/ROSA26 kit |
ROSA26 safe harbor gRNA vector, validated ROSA26 targeting sequence cloned in pCas-Guide vector
Function | Rosa26 gRNA |
pCas-Guide-CRISPRi vector, All-in-one Vector for CRISPR/Cas9 Gene Interference with dCas9-KRAB-MeCP2 and gRNA cloning site.
Function | All-in-one CRISPRi vector |
pCas-Guide-GFP-CRISPRa vector, All-in-one Vector for CRISPR/Cas9 Gene activation with tGFP reporter, dCas9-VP64 and gRNA cloning site.
Function | All-in-one CRISPRa SAM vector |
ROSA26 donor vector with ROSA26 homologous arms, tGFP expression, and Puro selection (10 ug)
Function | ROSA26 donor vector |
AAVS1 donor vector with AAVS1 homologous arms, tGFP under EF1a promoter, and Puro selection (10 ug)
Function | AAVS1 donor vector |
Donor vector with AAVS1 homologous arms, C-terminal mGFP tag, Puro resistance, ready for transgene cloning
Function | AAVS1 donor vector |
pCas-Guide-Puro-CRISPRi Vector, All-in-one Vector for CRISPR/Cas9 Gene Interference with Puro selection marker, dCas9-KRAB-MeCP2 and gRNA cloning site.