Products

Rabbit polyclonal IL-4 antibody Biotin Conjugated

Applications WB
Reactivities Human

Rabbit Polyclonal Anti-GAPDH Antibody (biotin)

Applications WB
Reactivities Human, Mouse, Rat, Rabbit, Chicken

Rabbit polyclonal IL-17A antibody Biotin Conjugated

Applications WB
Reactivities Human

Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/150,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions
ELISA: 1/20,000-1/100,000.
Immunohistochemistry: 1/1,000-1/5,000.
Western blot: 1/2,000-1/10,000.
Reactivities Mouse
Conjugation Biotin

Human IgG (gamma chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Alpha 1 Acid Glycoprotein (ORM1) rabbit polyclonal antibody, Biotin, Purified

Applications ELISA, WB
Reactivities Human

Luciferase (Photinus pyralis) goat polyclonal antibody, Biotin, Purified

Applications ELISA, WB

GST-Tag goat polyclonal antibody, Biotin

Applications ELISA, WB
Reactivities Schistosoma japonicum

Acid Phosphatase rabbit polyclonal antibody, Biotin, Purified

Applications ELISA, WB

Carbonic Anhydrase I (CA1) goat polyclonal antibody, Biotin, Purified

Applications ELISA, WB
Reactivities Human

pgmB goat polyclonal antibody, Biotin, Purified

Applications ELISA, WB
Reactivities Lactococcus lacti

pep1 rabbit polyclonal antibody, Biotin, Purified

Applications ELISA, WB
Reactivities Maize

KTI3 rabbit polyclonal antibody, Biotin

Applications ELISA, WB
Reactivities Lima Bean

CPB1 rabbit polyclonal antibody, Biotin, Purified

Applications ELISA, WB
Reactivities Porcine

Canine IgG (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Canine
Conjugation Biotin