Products

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.

Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Sheep IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.

Immunohistochemistry on Frozen Sections.
Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Sheep IgG (Fc specific) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used As unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5.000.
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated

Duck IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Antibody titre: Precipitin titre not less than 1/128 when tested against normal Duck serum in agar block titration.
Reactivities Duck
Conjugation Unconjugated

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC

Applications

FLISA.
Immunofluoresecence: ≤ 1 μg/106 cells.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation FITC

Mouse IgA (alpha chain specific) goat polyclonal antibody, Texas Red

Applications FLISA: 1/100-1/400.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation Texas Red

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation AP

Mouse IgA (alpha chain specific) goat polyclonal antibody, TRITC

Applications FLISA: 1/100-1/400.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation TRITC

Mouse IgA (alpha chain specific) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Texas Red

Applications

FLISA: 1/100–1/400.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation Texas Red

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC

Applications

FLISA: 1/100–1/400.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation TRITC

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP

Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Rabbit
Conjugation FITC

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Rabbit
Conjugation HRP