Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9
| Reactivities | Human |
| Conjugation | Unconjugated |
Goat anti-rabbit IgG Antiserum, pretitered, polyclonal
| Reactivities | Human, Mammalian |
| Conjugation | Unconjugated |
Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, Purified
| Applications | ELISA: 5 µ/ml as a coating antibody. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Human IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain) porcine polyclonal antibody, Serum
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Canine IgG (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Canine IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Human IgD (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Chicken IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Canine IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/3000. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Mouse IgG rabbit polyclonal antibody, Serum
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Human IgG rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Immunodiffusion. For Ouchterlony: Sample Size: 15 µl Distance between wells: 5 mm Test time: 4 days at 37°C Antigens tested: Normal human serum, purified human IgG, normal mouse serum. Results: Titre with NHS > 1:1000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction
| Applications | Immunoprecipitation. Immunodiffusion. Ouchterlony Test: Sample size: 15 µl. Distance between wells: 5 mm. Test Time: 5 days. Temperature: 37°C. Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG. Results: Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG. No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution. Protein content: Biuret Assay 14 mg/ml. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
