Purified recombinant protein of Human tyrosine kinase, non-receptor, 1 (TNK1), full length, with N-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Products
Purified recombinant protein of Human C-type lectin domain family 3, member A (CLEC3A), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human amphiregulin (AREG), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1 (GNAT1), transcript variant 2, full length, with N-terminal His tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human ets variant 5 (ETV5), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human forkhead box F1 (FOXF1), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1,Asp22-Leu119, with N-terminal His-ABP tag, expressed in E. coli, 50ug
| Tag | N-His-ABP (Albumin-Binding Protein) |
| Expression Host | E. coli |
Purified recombinant protein of Human ribosomal protein L3 (RPL3), transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human kelch-like 12 (Drosophila) (KLHL12), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human AT rich interactive domain 1A (SWI-like) (ARID1A), transcript variant 1, Asn1986-End, with N-terminal His tag, expressed in E.coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human F-box and leucine-rich repeat protein 5 (FBXL5), transcript variant 2, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human septin 7 (SEPT7), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5), His346-Pro622, with N-terminal His tag, expressed in E.coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform 1 precursor (BCKDHB), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), 1445Gly-1669Arg, with N-terminal His tag, expressed in E.coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
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Species
- Human (4752)
- Mouse (111)
- Rat (22)
- Escherichia coli (16)
- Blomia tropicalis (10)
- SARS-CoV-2 (8)
- Dermatophagoides pteronyssinus (6)
- Streptococcus Pyogenes (5)
- Staphylococcus aureus (4)
- Orf Virus (3)
- Bovine (2)
- Dermatophagoides farinae (2)
- Rabbit (2)
- Streptococcus sp. (2)
- Alternaria alternata (1)
- Betula pendula (1)
- E.coli (1)
- Enterobacteria phage T4 (1)
- Escherichia phage T7 (1)
- HIV (1)
- Hepatitis B Virus (1)
- Humulus japonicus (1)
- Lachnospiraceae bacterium ND2006 (1)
- Lepidoglyphus destructor (1)
- Leptotrichia wadei (1)
- Macaca fascicularis (1)
- Mucor hiemalis (1)
- Mus musculus (1)
- Saccharomyces cerevisiae (1)
- Serratia marcescens (1)
- Staphylococcus aureus subsp. (1)
- Streptomyces avidinii (1)
- Streptomyces plicatus (1)
- Tobacco etch virus (1)
- Vaccinia virus (1)
Target Symbol
- VEGFA (27)
- TNF ALPHA (15)
- FGF2 (13)
- IL6 (13)
- PTPRN (12)
- FGF1 (10)
- NFYA (10)
- BDNF (9)
- EGF (9)
- EOTAXIN 3 (9)
- IGF1 (9)
- IL4 (9)
- SDF1 (9)
- ALPHA SYNUCLEIN (8)
- BMP2 (8)
- FSTL1 (8)
- HIP2 (8)
- IL22 (8)
- IL33 (8)
- IL8 (8)
- PDGF AA (8)
- SUMO 3 (8)
- BNP (7)
- CSF2 (7)
- FABP4 (7)
- FERRITIN HEAVY CHAIN (7)
- IL1B (7)
- IL3 (7)
- LIF (7)
- TRAIL (7)
- BLNK (6)
- CNTF (6)
- GDNF (6)
- GLUTAMINE SYNTHETASE (6)
- IL1 BETA (6)
- MAP1LC3A (6)
- NEUROTROPHIN 3 (6)
- NHP2 (6)
- TGIF (6)
- TNF (6)
- TRIM72 (6)
- ADIPOQ (5)
- AMPHIREGULIN (5)
- ANNEXIN VI (5)
- APE1 (5)
- APOLIPOPROTEIN E (5)
- ASB13 (5)
- ASF1A (5)
- BETA ARRESTIN 1 (5)
- BID (5)
- BIRC5 (5)
- CALCITONIN (5)
- CASPASE 10 (5)
- CSPS (5)
- CXCL14 (5)
- EIF1A (5)
- EIF4EBP1 (5)
- FGF21 (5)
- FIBRONECTIN (5)
- GABARAPL1 (5)
- GADD45B (5)
- GLUTATHIONE TRANSFERASE ZETA 1 (5)
- HAO1 (5)
- IL15 (5)
- IL17F (5)
- IL1RA (5)
- IL7 (5)
- INTERFERON GAMMA (5)
- KGF (5)
- KLF6 (5)
- KPNA2 (5)
- LACTB2 (5)
- LEPTIN (5)
- LSM4 (5)
- LYSOPHOSPHOLIPASE 1 (5)
- MAX (5)
- MGMT (5)
- MYOZENIN 2 (5)
- NCK (5)
- NEDD8 (5)
- NEURITIN (5)
- NGAL (5)
- ONCOSTATIN M (5)
- PARATHYROID HORMONE (5)
- PDCD10 (5)
- PDGF BETA (5)
- PDGFA (5)
- PEROXIREDOXIN 4 (5)
- PGP9.5 (5)
- PIN4 (5)
- PKIB (5)
- SENP2 (5)
- SUMO 1 (5)
- SUPEROXIDE DISMUTASE 1 (5)
- SYNTAXIN 6 (5)
- TRYPTOPHANYL TRNA SYNTHETASE (5)
- TXN (5)
- UBE2G2 (5)
- UPB1 (5)
- YY1 (5)
Tags
- N-His (2724)
- Tag Free (595)
- C-His (437)
- N-GST and C-His (435)
- N-GST and C-HIS (150)
- N-GST (89)
- N-His-ABP (Albumin-Binding Protein) (52)
- His-tag (49)
- N-His&C-His (40)
- N-T7&C-His (24)
- N-Trx&His (20)
- N-His-Trx (18)
- N-GST, C-His (13)
- N-HIS (9)
- N-His&SUMO (8)
- Tag-free (8)
- N-6His (6)
- N-His PDCD1(Pro21-Val170) (5)
- N-GST&His (4)
- N-His&T7 (4)
- N-His-GST (4)
- N-Trx (4)
- C-Avi&6His (3)
- C-HIS (3)
- His-tagged, myc-tagged (3)
- N-Avi (3)
- Myc-DDK (2)
- N-His-PDCD1(Pro21-Val170) (2)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- C-DDK (1)
- C-DDK-His (1)
- C-HIS/C (1)
- C-Myc/DDK (1)
- DDK-tag (1)
- His tag (1)
- N-AVI (1)
- N-DDK (1)
- N-FUSION (1)
- N-GFP (1)
- N-GST(CKM) and N-His(CKB) (1)
- N-HIS and C-AVIPLUS (1)
- N-PROTEIN (1)
- N-T7 (1)
- N-TRX (1)
- N-TRXA (1)
- N-Trx-His (1)
- N-terminal His-tag (1)
Families
- Druggable Genome (2248)
- Secreted Protein (707)
- Transcription Factors (643)
- Transmembrane (642)
- ES Cell Differentiation/IPS (246)
- Protein Kinase (155)
- Stem cell - Pluripotency (155)
- Protease (133)
- Adult stem cells (87)
- Embryonic stem cells (75)
- Phosphatase (63)
- Induced pluripotent stem cells (41)
- Ion Channels: Other (40)
- Cancer stem cells (31)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (26)
- Stem cell relevant signaling - JAK/STAT signaling pathway (25)
- Stem cell relevant signaling - DSL/Notch pathway (15)
- Stem cell relevant signaling - Wnt Signaling pathway (15)
- 10 mM Tris-HCl (12)
- GPCR (12)
- Nuclear Hormone Receptor (12)
- the solution can be further dialyzed against PBS (phosphate buffered saline (12)
- 2 mM dithiothreitol (10)
-
ELISA.
Western Blot. (10) - e.g. Dulbecco's PBS). (10)
- Ion Channels: Potassium (8)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (8)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (6) - P450 (6)
- pH 7.4). For immunization purposes (6)
- Ion Channels: Transient receptor potential (4)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (4) -
pH 7.4).
For immunization purposes (4) -
pH 7.4).
For immunization purposes (4) - Ion Channels: Cys-loop Receptors (3)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies. (3) - Western Blot. (3)
- 0.1% (w/v) insulin (2)
- 10mM Tris-HCl (2)
- 1mM DTT (2)
- 2 mM dithiothreitol (2)
-
2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (2) - 2mM dithiothreitol (2)
- 63mM sodium phosphate (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) - Can be used as standard in a Sandwich ELISA. (2)
- Ion Channels: Sodium (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) -
Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (2) -
both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
- e.g.Dulbecco's PBS). (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
-
ELISA.
Western blotting. (1) -
Positive control for Western blot analysis.
Standard for ELISA. (1) -
Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T (1) -
Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A (1) - ELISA (1)
-
ELISA.
Western blotting. (1) - Forssmann WG (1)
- Hawkins JA (1)
- Hidaka Y (1)
- Ion Channels: ATP Receptors (1)
- Marx UC (1)
-
Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA (1) - Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21. (1)
- Neudecker P (1)
- Nourse A (1)
- Rosch P (1)
- Schulz A (1)
- Shimonishi Y (1)
-
Western Blot.
Protocol: 1. Lauber T (1) - Western Blotting. (1)
Pathways
- Metabolic pathways (362)
- Cytokine-cytokine receptor interaction (315)
- Pathways in cancer (294)
- MAPK signaling pathway (216)
- Jak-STAT signaling pathway (145)
- Chemokine signaling pathway (124)
- Hematopoietic cell lineage (118)
- Regulation of actin cytoskeleton (118)
- Focal adhesion (114)
- Melanoma (105)
- Apoptosis (95)
- Natural killer cell mediated cytotoxicity (91)
- Toll-like receptor signaling pathway (91)
- T cell receptor signaling pathway (90)
- NOD-like receptor signaling pathway (82)
- Ubiquitin mediated proteolysis (81)
- Alzheimer's disease (76)
- Prostate cancer (73)
- Systemic lupus erythematosus (72)
- Fc epsilon RI signaling pathway (70)
- Cell cycle (69)
- TGF-beta signaling pathway (65)
- Neurotrophin signaling pathway (64)
- Huntington's disease (61)
- Type I diabetes mellitus (61)
- Wnt signaling pathway (60)
- ErbB signaling pathway (59)
- Hypertrophic cardiomyopathy (HCM) (59)
- Parkinson's disease (57)
- p53 signaling pathway (57)
- Amyotrophic lateral sclerosis (ALS) (55)
- Glioma (55)
- RIG-I-like receptor signaling pathway (55)
- Graft-versus-host disease (54)
- Cytosolic DNA-sensing pathway (53)
- Oocyte meiosis (52)
- Renal cell carcinoma (51)
- Acute myeloid leukemia (50)
- Dilated cardiomyopathy (50)
- Insulin signaling pathway (50)
- Pancreatic cancer (49)
- PPAR signaling pathway (48)
- Adipocytokine signaling pathway (47)
- Prion diseases (47)
- Asthma (46)
- Small cell lung cancer (46)
- Melanogenesis (45)
- Pyrimidine metabolism (45)
- Colorectal cancer (44)
- Gap junction (44)
- Chronic myeloid leukemia (43)
- mTOR signaling pathway (43)
- B cell receptor signaling pathway (42)
- Endocytosis (42)
- Bladder cancer (41)
- Allograft rejection (40)
- Hedgehog signaling pathway (39)
- Purine metabolism (38)
- Ribosome (38)
- Nitrogen metabolism (37)
- Glycolysis / Gluconeogenesis (36)
- Leukocyte transendothelial migration (35)
- Progesterone-mediated oocyte maturation (35)
- Type II diabetes mellitus (35)
- Arginine and proline metabolism (34)
- Basal cell carcinoma (34)
- Spliceosome (34)
- Axon guidance (33)
- Antigen processing and presentation (32)
- VEGF signaling pathway (32)
- Calcium signaling pathway (31)
- Epithelial cell signaling in Helicobacter pylori infection (30)
- Regulation of autophagy (30)
- Adherens junction (29)
- Vascular smooth muscle contraction (29)
- Endometrial cancer (28)
- GnRH signaling pathway (28)
- Long-term potentiation (28)
- Amino sugar and nucleotide sugar metabolism (25)
- Autoimmune thyroid disease (25)
- Oxidative phosphorylation (25)
- Fructose and mannose metabolism (24)
- Base excision repair (23)
- Complement and coagulation cascades (23)
- Drug metabolism - other enzymes (23)
- Glutathione metabolism (23)
- Neuroactive ligand-receptor interaction (23)
- Porphyrin and chlorophyll metabolism (23)
- Cell adhesion molecules (CAMs) (22)
- ECM-receptor interaction (22)
- Non-small cell lung cancer (22)
- Long-term depression (21)
- Metabolism of xenobiotics by cytochrome P450 (21)
- Tryptophan metabolism (21)
- Fc gamma R-mediated phagocytosis (20)
- Primary immunodeficiency (20)
- SNARE interactions in vesicular transport (20)
- Glycerophospholipid metabolism (19)
- Sulfur metabolism (19)
- Cardiac muscle contraction (18)
- Glycine (18)
- Pyruvate metabolism (18)
- RNA degradation (18)
- Tyrosine metabolism (18)
- serine and threonine metabolism (18)
- Cysteine and methionine metabolism (17)
- Pathogenic Escherichia coli infection (17)
- Inositol phosphate metabolism (15)
- Phenylalanine metabolism (15)
- Thyroid cancer (15)
- Tight junction (15)
- ABC transporters (14)
- Alanine (14)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (14)
- Terpenoid backbone biosynthesis (14)
- Viral myocarditis (14)
- aspartate and glutamate metabolism (14)
- Glyoxylate and dicarboxylate metabolism (13)
- Phosphatidylinositol signaling system (13)
- Propanoate metabolism (13)
- Retinol metabolism (13)
- Aminoacyl-tRNA biosynthesis (12)
- Androgen and estrogen metabolism (12)
- Citrate cycle (TCA cycle) (12)
- Dorso-ventral axis formation (12)
- Drug metabolism - cytochrome P450 (12)
- Arachidonic acid metabolism (11)
- Basal transcription factors (11)
- Fatty acid metabolism (11)
- Mismatch repair (11)
- Notch signaling pathway (11)
- Nucleotide excision repair (11)
- Olfactory transduction (11)
- Pantothenate and CoA biosynthesis (11)
- Valine (10)
- beta-Alanine metabolism (10)
- Ether lipid metabolism (9)
- Galactose metabolism (9)
- Homologous recombination (9)
- Lysine degradation (9)
- Lysosome (9)
- Maturity onset diabetes of the young (9)
- Methane metabolism (9)
- Nicotinate and nicotinamide metabolism (9)
- Pentose and glucuronate interconversions (9)
- Pentose phosphate pathway (9)
- Proteasome (9)
- leucine and isoleucine degradation (9)
- DNA replication (8)
- One carbon pool by folate (8)
- Renin-angiotensin system (8)
- Selenoamino acid metabolism (8)
- Ubiquinone and other terpenoid-quinone biosynthesis (8)
- Butanoate metabolism (7)
- N-Glycan biosynthesis (7)
- Riboflavin metabolism (7)
- Steroid biosynthesis (6)
- Vibrio cholerae infection (6)
- C21-Steroid hormone metabolism (5)
- Glycosphingolipid biosynthesis - lacto and neolacto series (5)
- Heparan sulfate biosynthesis (5)
- Primary bile acid biosynthesis (5)
- Sphingolipid metabolism (5)
- Synthesis and degradation of ketone bodies (5)
- Chondroitin sulfate biosynthesis (4)
- D-Arginine and D-ornithine metabolism (4)
- Histidine metabolism (4)
- Keratan sulfate biosynthesis (4)
- Linoleic acid metabolism (4)
- Non-homologous end-joining (4)
- O-Glycan biosynthesis (4)
- Starch and sucrose metabolism (4)
- Taste transduction (4)
- Taurine and hypotaurine metabolism (4)
- alpha-Linolenic acid metabolism (4)
- Circadian rhythm - mammal (3)
- Folate biosynthesis (3)
- RNA polymerase (3)
- Caffeine metabolism (2)
- D-Glutamine and D-glutamate metabolism (2)
- Glycosaminoglycan degradation (2)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (2)
- Limonene and pinene degradation (2)
- Other glycan degradation (2)
- Phenylalanine (2)
- leucine and isoleucine biosynthesis (2)
- tyrosine and tryptophan biosynthesis (2)
- Ascorbate and aldarate metabolism (1)
- Biosynthesis of unsaturated fatty acids (1)
- Cyanoamino acid metabolism (1)
- Fatty acid elongation in mitochondria (1)
- Glycerolipid metabolism (1)
- Glycosphingolipid biosynthesis - ganglio series (1)
- Lipoic acid metabolism (1)
- Protein export (1)
