Products

Antibodies

cDNA Clones

Proteins

Primary Antibodies

Expression Plasmids

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant ORF Clones

Native Proteins

Human

Mouse

Humon

BETA CATENIN

EGFR

ALPHA 1 ANTITRYPSIN

BDNF

AKT1

GFAP

CD44

CD31

HNF 4 ALPHA

CD4

CD56

JNK1

CD45

CD117

C-JUN

BCL2

E CADHERIN

CD147

L1CAM

CD133

KI67

CD8A

CD32A

JNK2

CD105

PI 3 KINASE CATALYTIC SUBUNIT GAMMA

CD16

CASPASE 3

M-CSF

P GLYCOPROTEIN

EPCAM

MAP2

GAPDH

PAX3

CD34

CDCP1

ID3

IGF1

TCF4

TTF1

CD14

VIMENTIN

NEUROFILAMENT

G CSF

HGF

LDL RECEPTOR

VCAM1

C-MYB

CDX2

GLI1

CD95

HES1

PDGF RECEPTOR BETA

PTCH1

CD32B

EGF

INTEGRIN BETA 1

SMAD2

GATA3

ICAM2

PVRL1

ER81

CARDIAC TROPONIN I

CYSTATIN C

MANNOSE PHOSPHATE ISOMERASE

AKT2

AKT3

CYTOKERATIN 7

NEUROGENIN 1

PDGF RECEPTOR ALPHA

SMAD1

CD70

FGF21

PRION PROTEIN PRP

SRC

CD244

CD30

PROCR

SYNDECAN 1

TAL1

AMINOPEPTIDASE A

CD13

CD43

STAT5A

ERK1

IL6R

UPA

GM CSF

SMAD3

THYROID PEROXIDASE

ACTIVIN RECEPTOR TYPE IA

CD71

HOXD8

N CADHERIN

INSULIN

STAT6

BCL X

CKII ALPHA

FIBRONECTIN

IL2 RECEPTOR ALPHA

Human

Mouse

Rat

Dog

Monkey

Bovine

Canine

Primate

Human (Expected from sequence similarity: Mouse

Pig

Chicken

Cow)

Porcine

Human (Predicted: Mouse

Rabbit

Hamster

Feline

Zebrafish

Pig)

Sheep

Bat

Dog)

Rat)

Gorilla

Bovine)

Human (Predicted: Monkey)

Guinea Pig

Human (Predicted: Mouse)

Xenopus)

Rabbit)

Xenopus

Goat

Chimpanzee

Cow

Drosophila

Horse)

Human (Expected from sequence similarity: Dog)

Mouse (Expected from sequence similarity: Rat

Rat (Expected from sequence similarity: Dog

Avian

Equine

Gibbon

Gibbon (Predicted: Monkey

Horse

Human (Expected from sequence similarity: Dog

Human (Predicted: Bat

Human (Predicted: Chicken)

Monkey)

Mouse (Predicted: Rat

Rat (Expected from sequence similarity: Dog)

X. tropicalis)

All Species

Fish

Guinea Pig)

Human (Predicted: Bovine

Macaque

Mammalian

Mouse (Predicted: Chicken

Opossum

Pig (Expected from sequence similarity: Dog)

Rat (Predicted: Mouse)

X. tropicalis

Zebrafish)

Broad

C. elegans (Does not react with: Human

Drosophila)

Gerbil

Gibbon (Predicted: Dog

Guinea Pig (Expected from sequence similarity: Dog

Hamster)

Horse (Predicted: Bovine

Horse (Predicted: Monkey

Human (Expected from sequence similarity: Cow)

Human (Expected from sequence similarity: Mouse)

Human (Expected from sequence similarity: Pig

Human (Expected from sequence similarity: Rat

Human (Predicted: Bovine)

Human (Predicted: Dog

Human (Predicted: Rat

Human (Predicted: Rhesus Monkey)

Mouse (Does not react with: Rabbit)

Mouse (Expected from sequence similarity: Dog

Mouse (Predicted: Dog)

Mouse (Predicted: Feline

Mouse (Predicted: Rat)

Mouse (Predicted: Rhesus Monkey)

Mouse (Predicted: Zebrafish

Mouse)

Orang-Utan (Predicted: Dog

Pig (Expected from sequence similarity: Feline

Pig (Expected from sequence similarity: Human

Pig (Expected from sequence similarity: Rat

Protozoa

Quail

Rat (Expected from sequence similarity: Human)

Rat (Predicted: Bovine

Rhesus Monkey

Yeast

WB

IHC

IF

FC

ELISA

IP

ICC/IF

LMNX

10k-ChIP

Simple Western

FN

Dot

PEP-ELISA

Immunoblotting

CyTOF-ready

ChIP

ICC

Electron Microscopy

R

Block/Neutralize

SISCAPA

Mouse

Rabbit

Goat

Rat

Chicken

Sheep

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Baculovirus

Plasmids

Myc-DDK

mGFP

C-Myc/DDK

Tag Free

TurboGFP

C-MYC/DDK

N-His

C-His

C-DDK

DDK-His

N-GST and C-His

C-DDK/His

N-GST and C-HIS

His-tag

C-6His

C-Fc

C-6×His

His-Tag

N-His-ABP (Albumin-Binding Protein)

C-8His

C-Human Fc

C-hFc

N-6His

N-His-Trx

C- 6×His

C-HIS

C-Human Fc and 6×His

C-Mouse Fc

C-Mouse Fc and 6×His

Tag-free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-XL5-DDK-His

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

APC

PerCP

APC-Cy7

ATTO 655

Cy5

DyLight 488

PE-Cy5.5

PE-Cy7

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG3

IgY

IgG2c

IgG2a, kappa

IgG2b, kappa

IgG, kappa

IgM

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

TrueMAB

LentiORF

TrueORF

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Ion Channels: Potassium

Induced pluripotent stem cells

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Pathways in cancer

Focal adhesion

Colorectal cancer

Cytokine-cytokine receptor interaction

MAPK signaling pathway

Cell adhesion molecules (CAMs)

Hematopoietic cell lineage

Prostate cancer

Regulation of actin cytoskeleton

Wnt signaling pathway

Melanoma

ErbB signaling pathway

Endometrial cancer

Pancreatic cancer

Adherens junction

Jak-STAT signaling pathway

T cell receptor signaling pathway

Leukocyte transendothelial migration

Neurotrophin signaling pathway

Glioma

Basal cell carcinoma

Acute myeloid leukemia

Endocytosis

Fc gamma R-mediated phagocytosis

Toll-like receptor signaling pathway

Melanogenesis

TGF-beta signaling pathway

Natural killer cell mediated cytotoxicity

Tight junction

Progesterone-mediated oocyte maturation

Non-small cell lung cancer

Small cell lung cancer

Fc epsilon RI signaling pathway

Insulin signaling pathway

GnRH signaling pathway

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Chronic myeloid leukemia

Apoptosis

Epithelial cell signaling in Helicobacter pylori infection

Renal cell carcinoma

B cell receptor signaling pathway

Gap junction

Pathogenic Escherichia coli infection

Hedgehog signaling pathway

VEGF signaling pathway

Chemokine signaling pathway

Adipocytokine signaling pathway

Maturity onset diabetes of the young

mTOR signaling pathway

ECM-receptor interaction

Hypertrophic cardiomyopathy (HCM)

Metabolic pathways

Thyroid cancer

Type II diabetes mellitus

Dilated cardiomyopathy

Bladder cancer

Calcium signaling pathway

Primary immunodeficiency

Systemic lupus erythematosus

Notch signaling pathway

Amyotrophic lateral sclerosis (ALS)

Alzheimer's disease

Axon guidance

Dorso-ventral axis formation

Huntington's disease

Prion diseases

Antigen processing and presentation

NOD-like receptor signaling pathway

Complement and coagulation cascades

RIG-I-like receptor signaling pathway

Viral myocarditis

p53 signaling pathway

Autoimmune thyroid disease

Oocyte meiosis

Long-term depression

Cell cycle

Renin-angiotensin system

Type I diabetes mellitus

Phosphatidylinositol signaling system

Glycolysis / Gluconeogenesis

Allograft rejection

ABC transporters

Long-term potentiation

Vascular smooth muscle contraction

Vibrio cholerae infection

Lysosome

Inositol phosphate metabolism

Graft-versus-host disease

Neuroactive ligand-receptor interaction

Parkinson's disease

Tyrosine metabolism

Nicotinate and nicotinamide metabolism

Cardiac muscle contraction

Amino sugar and nucleotide sugar metabolism

Fructose and mannose metabolism

Retinol metabolism

Glutathione metabolism

Regulation of autophagy

Starch and sucrose metabolism

PPAR signaling pathway

Purine metabolism

Pyrimidine metabolism

Drug metabolism - cytochrome P450

Drug metabolism - other enzymes

Linoleic acid metabolism

Metabolism of xenobiotics by cytochrome P450

Citrate cycle (TCA cycle)

Pyruvate metabolism

Asthma

Cysteine and methionine metabolism

Phenylalanine

Phenylalanine metabolism

Ubiquinone and other terpenoid-quinone biosynthesis

tyrosine and tryptophan biosynthesis

Galactose metabolism

Oxidative phosphorylation

Ubiquitin mediated proteolysis

Primary bile acid biosynthesis

Human 293

E. coli

Human

Plasma

96-well plate, Luminex

Luminex

96-well plate

Neomycin

Puromycin

None

Recombinant Antibodies

TA600050

TA600277

TA600279

TA700050

TA700170

TA700245

TA600055

TA700218

TA700239

TA700278

TA700279

TA700391

TA700469

TA700484

TA700515

TA500337, TA600040

TA600003

TA600004

TA600005

TA600007

TA600009

TA600010

TA600017

TA600034

TA600035, TA600034

TA600035, TA600047, TA600141

TA600036

TA600037

TA600040

TA600040, TA600039

TA600041

TA600047

TA600053

TA600103, TA600104

TA600104, TA600103

TA600105

TA600106

TA600107

TA600108

TA600170, TA600171, TA600172

TA600173

TA600196

TA600197

TA600218, TA600220, TA600221

TA600219

TA600221

TA600239, TA600240

TA600244, TA600245, TA600247

TA600244, TA600246, TA600249, TA600250

TA600247, TA600248

TA600248

TA600253

TA600255

TA600278, TA600280

TA600281, TA600282

TA600389

TA600391, TA600393

TA600469, TA600472

TA600470

TA600471

TA600473

TA600474

TA600482

TA600482, TA600484

TA600483, TA600485, TA600484

TA600515, TA600516

TA600533

TA600533, TA600534

TA600541

TA600542

TA600543

TA600544

TA600559

TA700003

TA700004

TA700005

TA700007

TA700009

TA700010

TA700017

TA700034, TA700035

TA700034, TA700141

TA700035

TA700035, TA700047

TA700036

TA700037

TA700039

TA700039, TA700040, TA700078

TA700040

TA700041

TA700055, TA700077

TA700103, TA700104

TA700104, TA700103

TA700105

TA700106

TA700107

TA700108

TA700171

TA700196

TA700197

TA700218, TA700220

TA700219

TA700244

TA700244, TA700245

TA700244, TA700246

TA700246, TA700247

TA700277, TA700298, TA700300

TA700297, TA700299, TA700301

TA700389

TA700390

TA700470

TA700471

TA700472

TA700473

TA700482, TA700483

TA700483, TA700484

TA700533, TA700534

TA700534

TA700542

TA700543

TA700544

TA700545

TA700559

Carrier-free Antibodies

1

2

3

Yes

In Stock