Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant ORF Clones

Mutant Proteins

Native Proteins

Lentiviral Particles

Over-expression Lysates

Human

Mouse

EGFR

ALPHA 1 ANTITRYPSIN

BDNF

VEGFA

G PROTEIN ALPHA S

NRG1

CD163

FGFR2

EMA

GALECTIN 3

GM CSF RECEPTOR ALPHA

KALLIKREIN 8

PERIOSTIN

CD8A

SERPINB3

FACTOR XIIIA

ERBB 3

OSTEOPONTIN

ALPHA DYSTROGLYCAN

IL32

LOX

FGF1

CD16

IL1RA

APOLIPOPROTEIN E

HAPTOGLOBIN

M-CSF

TNF ALPHA

AGR2

ANNEXIN A2

FSH BETA

IGF1

MESOTHELIN

G CSF

BNP

IL6

APOLIPOPROTEIN A V

MIDKINE

RNASE9

SEX HORMONE BINDING GLOBULIN

ADIPONECTIN

INTERFERON GAMMA

SPINT1

ARTS1

CD95

HE4

APOLIPOPROTEIN L 1

LIPG

MTMR14

TIMP2

AMH

BMP4

PROLACTIN

RAB37

VEGF RECEPTOR 1

ANGIOTENSIN CONVERTING ENZYME 2

HSD17B13

PROSTATE SPECIFIC ANTIGEN

CLUSTERIN

SERPINE2

CD40

MSMB

SIL1

ALBUMIN

ALPHA 1 FETOPROTEIN

FIBRINOGEN GAMMA CHAIN

GRANULIN

SYNAPTOTAGMIN 1

CD23

CHROMOGRANIN A

FGF21

PARATHYROID HORMONE RELATED PROTEIN

SERPINB2

ST3GAL3

TNFRSF18

BETA 2 MICROGLOBULIN

FGF2

GROWTH HORMONE

IL17

IL1RAP

IL2

IL24

KALLIKREIN 11

PLAUR

SIGLEC6

SYNAPTOPHYSIN

WNT3

AMCASE

LAMININ 5

NEUROPILIN 1

CALCITONIN

CALRETICULIN

FACTOR I

GM CSF

PARATHYROID HORMONE

CD71

DECORIN

EFEMP1

FOLR2

IL12A

Human

Mouse

Rat

Monkey

Dog

Bovine

Human (Expected from sequence similarity: Mouse

Pig

Porcine

Canine

Chicken

Rabbit

Cow)

Human (Predicted: Mouse

Bovine)

Dog)

Gorilla

Primate

Hamster

Bat

Pig)

Rat)

Cow

Guinea Pig

Horse

Human (Expected from sequence similarity: Dog)

Rabbit)

Gibbon

Macaque

Equine

Human (Predicted: Monkey)

Mouse (Expected from sequence similarity: Rat

Feline

Human (Predicted: Monkey

Sheep

Human (Expected from sequence similarity: Cow)

Zebrafish

Chimpanzee

Human (Predicted: Pig

Gibbon (Predicted: Mouse

Goat

Horse)

Human (Expected from sequence similarity: Dog

Human (Expected from sequence similarity: Rat

Human (Predicted: Bovine)

Mammalian

Mouse (Expected from sequence similarity: Rat)

Xenopus

Xenopus)

Expected from sequence similarity: Human

Fish

Hamster)

Human (Expected from sequence similarity: Mouse)

Human (Predicted: Chimpanzee)

Human (Predicted: Dog

Human (Predicted: Mouse)

Human (Predicted: Rabbit)

Human (Predicted: Rat

Mouse (Predicted: Chicken

Mouse (Predicted: Rhesus Monkey)

Opossum

Rat (Expected from sequence similarity: Dog

Amphibian

Bear

Birds

Chicken)

Gibbon (Predicted: Hamster)

Gibbon (Predicted: Monkey

Gibbon (Predicted: Monkey)

Gibbon (Predicted: Rat

Guinea Pig)

Horse (Predicted: Bat

Horse (Predicted: Bovine

Horse (Predicted: Monkey

Horse (Predicted: Monkey)

Human (Expected from sequence similarity: Pig)

Human (Predicted: Bat)

Human (Predicted: Bovine

Human (Predicted: Chicken)

Human (Predicted: Dog)

Human (Predicted: Hamster)

Human (Predicted: Horse

Human (Predicted: Pig)

Human (Predicted: Rat)

Human (Predicted: Rhesus Monkey)

Monkey)

Mouse (Does not react with: Rat)

Mouse (Predicted: Dog

Mouse (Predicted: Horse

Mouse (Predicted: Monkey)

Mouse (Predicted: Rabbit

Mouse (Predicted: Rat

Mouse)

Orang-Utan

Orang-Utan (Predicted: Bat

Orang-Utan (Predicted: Horse)

Orang-Utan (Predicted: Monkey)

Pig (Expected from sequence similarity: Cow)

Pig (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Cow)

Rat (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Human

Rat (Expected from sequence similarity: Mouse

Rat (Expected from sequence similarity: Pig

Rhesus Monkey

Rhesus Monkey)

SimianIVE

Zebrafish)

WB

IHC

IF

ELISA

FC

ICC/IF

LMNX

IP

FN

10k-ChIP

Simple Western

ID

R

PEP-ELISA

Dot

FACS

CyTOF-ready

SISCAPA

Block/Neutralize

Electron Microscopy

ICC

Immunoblotting

Neutralize

Ie

LF

Labeling

Protein Array

SDS-PAGE

Mouse

Rabbit

Goat

Rat

Sheep

Chicken

Guinea Pig

HEK293T

HEK293

E. coli

Insect

CHO

Sf9

Baculovirus

Pichia

Plasmids

Particles

Myc-DDK

C-Myc/DDK

mGFP

Tag Free

TurboGFP

C-MYC/DDK

N-His

C-His

C-6His

N-GST and C-His

N-GST and C-HIS

C-DDK

C-HIS

C-DDK/His

C-8His

DDK-His

His-tag

C-Human Fc

His-Tag

N-His-Trx

N-His-ABP (Albumin-Binding Protein)

Tag-free

C-6×His

N-Avi

C-Fc

C-hFc

N-6His

N-His/DDK

C-10His

N-8His

N-His-PDCD1(Pro21-Val170)

C-6xHis/AVI

C-Avi&6His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FLAG-Fc

C-Fc/His

C-Human Fc and 6×His

C-Mouse Fc

C-terminal His

N-DDK/His

N-FLAG

N-Fc-FLAG

N-HIS

N-T7

N-Trx-His

N-hFc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

Tag free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-XL5-DDK-His

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

Peroxidase

APC

APC-Cy7

Cy5

Magnetic Beads

PE-Cy5.5

PE-Cy7

PerCP

TRITC

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgM

IgG2b, kappa

IgG2a, kappa

IgG3

IgY

IgG3, kappa

IgM, kappa

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

LentiORF

TrueMAB

TrueORF

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Induced pluripotent stem cells

Ion Channels: Potassium

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Transient receptor potential

Ion Channels: Glutamate Receptors

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Calcium

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Cytokine-cytokine receptor interaction

Pathways in cancer

Jak-STAT signaling pathway

MAPK signaling pathway

Complement and coagulation cascades

Focal adhesion

Hematopoietic cell lineage

Regulation of actin cytoskeleton

Melanoma

Toll-like receptor signaling pathway

Metabolic pathways

Chemokine signaling pathway

TGF-beta signaling pathway

GnRH signaling pathway

Natural killer cell mediated cytotoxicity

ECM-receptor interaction

Pancreatic cancer

Melanogenesis

Hedgehog signaling pathway

Bladder cancer

Autoimmune thyroid disease

Type I diabetes mellitus

Allograft rejection

Endocytosis

Prostate cancer

Systemic lupus erythematosus

T cell receptor signaling pathway

Wnt signaling pathway

Neuroactive ligand-receptor interaction

Basal cell carcinoma

ErbB signaling pathway

RIG-I-like receptor signaling pathway

Dilated cardiomyopathy

Cytosolic DNA-sensing pathway

Antigen processing and presentation

Calcium signaling pathway

Graft-versus-host disease

Hypertrophic cardiomyopathy (HCM)

NOD-like receptor signaling pathway

mTOR signaling pathway

Renal cell carcinoma

Gap junction

Long-term depression

Fc epsilon RI signaling pathway

Asthma

Apoptosis

Prion diseases

Epithelial cell signaling in Helicobacter pylori infection

PPAR signaling pathway

Alzheimer's disease

VEGF signaling pathway

Regulation of autophagy

Vascular smooth muscle contraction

Adipocytokine signaling pathway

Glioma

Colorectal cancer

Axon guidance

Neurotrophin signaling pathway

Primary immunodeficiency

p53 signaling pathway

Cell adhesion molecules (CAMs)

Non-small cell lung cancer

Small cell lung cancer

Adherens junction

Dorso-ventral axis formation

Endometrial cancer

Renin-angiotensin system

Arachidonic acid metabolism

Type II diabetes mellitus

Glycerolipid metabolism

Ether lipid metabolism

Viral myocarditis

Huntington's disease

Taste transduction

Vibrio cholerae infection

Glycerophospholipid metabolism

Fc gamma R-mediated phagocytosis

Linoleic acid metabolism

alpha-Linolenic acid metabolism

Amyotrophic lateral sclerosis (ALS)

Oocyte meiosis

Progesterone-mediated oocyte maturation

Cell cycle

SNARE interactions in vesicular transport

Glycosphingolipid biosynthesis - lacto and neolacto series

Glycosaminoglycan degradation

Chronic myeloid leukemia

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Keratan sulfate biosynthesis

Lysosome

Amino sugar and nucleotide sugar metabolism

Maturity onset diabetes of the young

Proteasome

O-Glycan biosynthesis

Purine metabolism

Pyrimidine metabolism

Histidine metabolism

Starch and sucrose metabolism

Insulin signaling pathway

Glutathione metabolism

Leukocyte transendothelial migration

N-Glycan biosynthesis

beta-Alanine metabolism

Other glycan degradation

Glycosphingolipid biosynthesis - ganglio series

Glycosphingolipid biosynthesis - globo series

Pathogenic Escherichia coli infection

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Tyrosine metabolism

Acute myeloid leukemia

Biotin metabolism

Arginine and proline metabolism

Galactose metabolism

Nitrogen metabolism

Tryptophan metabolism

Folate biosynthesis

Riboflavin metabolism

Human 293

Human

E. coli

Plasma

HEK293

P.Pichia

Pancreas

Pituitary

96-well plate, Luminex

96-well plate

Luminex

Neomycin

Puromycin

None

Recombinant Antibodies

TA600235

TA600400

TA700050

TA700083

TA700200

TA700202

TA700365

TA700399

TA700476

TA600097

TA600493

TA700029

TA700084

TA700089

TA700235

TA700329

TA700331

TA700364

TA700445

TA700469

TA600007

TA600014

TA600015

TA600016

TA600017

TA600021

TA600022

TA600023

TA600024

TA600025

TA600026

TA600027

TA600029, TA600043

TA600050

TA600056

TA600083

TA600084

TA600085, TA600086, TA600093

TA600087, TA600091

TA600088, TA600089

TA600090

TA600092

TA600094

TA600095

TA600096

TA600112

TA600113

TA600114

TA600145

TA600200

TA600200, TA600201, TA600202, TA600203

TA600204

TA600204, TA600205, TA600206, TA600207

TA600235, TA600236, TA600237, TA600238

TA600236

TA600329

TA600329, TA600332, TA600333

TA600330, TA600331

TA600364, TA600368

TA600365, TA600366, TA600367

TA600369

TA600370

TA600399, TA600401, TA600402

TA600445

TA600445, TA600446, TA600447

TA600456, TA600457

TA600457, TA600458, TA600570

TA600458

TA600469, TA600472

TA600470

TA600471

TA600473

TA600474

TA600476, TA600477, TA600478

TA600494

TA600494, TA600495

TA600549

TA600552

TA600553

TA600555, TA600556

TA600555, TA700556

TA600556, TA700556

TA600562

TA600564

TA600565

TA600566

TA600570

TA600571

TA700007

TA700014

TA700015

TA700016

TA700017

TA700021

TA700022

TA700023

TA700024

TA700025

TA700026

TA700027

TA700057

TA700085

TA700086

TA700087

TA700088

TA700094, TA700096

TA700095

TA700097

TA700112

TA700113

TA700114

TA700133

TA700145

TA700200, TA700201

TA700202, TA700203

TA700235, TA700236, TA700237, TA700238, TA700242

TA700235, TA700243

TA700329, TA700330

TA700366

TA700367

TA700400, TA700401, TA700402

TA700445, TA700446

TA700456

TA700456, TA700457

TA700457, TA700458

TA700470

TA700471

TA700472

TA700473

TA700493, TA700494

TA700495

TA700495, TA700501

TA700549

TA700552

TA700553

TA700554, TA700555

TA700555, TA600554

TA700562

TA700564

TA700565

TA700566

TA700570, TA700457

TA700571

Carrier-free Antibodies

1

2

3

Yes

In Stock