Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant ORF Clones

Mutant Proteins

Native Proteins

Lentiviral Particles

Over-expression Lysates

Human

Mouse

BDNF

VEGFA

NRG1

ALPHA 1 ANTITRYPSIN

G PROTEIN ALPHA S

EMA

EGFR

FGF1

ST3GAL3

FGFR2

ALPHA DYSTROGLYCAN

GM CSF RECEPTOR ALPHA

IL32

GELSOLIN

HYAL1

DECORIN

PACE4

ANNEXIN A2

IGF1

M-CSF

MTLRP

OSTEOPONTIN

RNASE9

IL1RA

PERIOSTIN

IL1RAP

FCRLM1

KALLIKREIN 11

ELASTIN

G CSF

GDNF

IL18BP

MIDKINE

SDF1

SEX HORMONE BINDING GLOBULIN

SPAG11B

CD16

FGF13

GROWTH HORMONE

KALLIKREIN 6

RAGE

THROMBOPOIETIN

FGL1

NEUROPILIN 1

PLAUR

IL37

PARATHYROID HORMONE RELATED PROTEIN

SIGLEC6

CALUMENIN

FAM3A

MAGP1

CD8B

TCTN1

AMCASE

CLUSTERIN

CRISP2

ERBB 3

HSD17B13

KALLIKREIN 8

MTMR14

RAB37

SYNAPTOTAGMIN 1

APOLIPOPROTEIN E

APRIL

CALCITONIN

CD95

NBL1

RIBONUCLEASE A

FGF8

IGF2

NELL2

PROSTATE SPECIFIC ANTIGEN

REG3A

RTBDN

EFEMP1

ENDOTHELIN 3

LEPTIN RECEPTOR

MESOTHELIN

REG3G

RNASE4

SPOCK3

VEGF RECEPTOR 1

C2

CDW75

ICAM4

LIPF

TRYPSIN

VMO1

APOER2

APOLIPOPROTEIN L 1

CD164

CTRP1

FIBRONECTIN

KININOGEN 1

MEPE

BMP4

CCL14

DKK3

EDA

IL4

IHC

WB

HEK293T

HEK293

E. coli

Insect

CHO

Sf9

Pichia

Plasmids

Particles

Myc-DDK

Tag Free

mGFP

C-Myc/DDK

TurboGFP

C-MYC/DDK

C-His

N-His

C-6His

C-Fc

N-GST and C-His

N-GST and C-HIS

C-DDK

C-HIS

C-Fc&His

C-DDK/His

His-tag

C-8His

N-GST

DDK-His

C-Human Fc

N-His-Trx

C-10His

C-mFc

N-6His

C-6His-Avi

N-His-ABP (Albumin-Binding Protein)

Tag-free

C-6×His

N-Avi

N-Fc

C-Avi-6His

C-hFc

N-His/DDK

N-8His

N-His-PDCD1(Pro21-Val170)

C-6xHis/AVI

C-AVI

C-AVI&His

C-Avi

C-Avi&6His

C-Avi-10His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-Fc-Avi

C-Fc/His

C-Flag,C-6His

C-His&Flag

C-His&HA&AVI

C-Human Fc and 6×His

C-Mouse Fc

C-hIgG2 Fc

C-hIgG4 Fc

C-terminal His

N-6His-Avi

N-AVI&His

N-DDK/His

N-FLAG

N-Fc-FLAG

N-Flag&His

N-HIS

N-His&C-His

N-His&Flag

N-T7

N-Trx-His

N-hFc

N-mFc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

Tag free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-XL5-DDK-His

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

Cytokine-cytokine receptor interaction

Pathways in cancer

Jak-STAT signaling pathway

MAPK signaling pathway

Complement and coagulation cascades

Regulation of actin cytoskeleton

Focal adhesion

Hematopoietic cell lineage

Chemokine signaling pathway

Metabolic pathways

Melanoma

Toll-like receptor signaling pathway

TGF-beta signaling pathway

ECM-receptor interaction

GnRH signaling pathway

Natural killer cell mediated cytotoxicity

Melanogenesis

Autoimmune thyroid disease

Pancreatic cancer

Hedgehog signaling pathway

Bladder cancer

Cytosolic DNA-sensing pathway

Wnt signaling pathway

Neuroactive ligand-receptor interaction

Dilated cardiomyopathy

RIG-I-like receptor signaling pathway

Systemic lupus erythematosus

Basal cell carcinoma

Prostate cancer

Endocytosis

Antigen processing and presentation

ErbB signaling pathway

Type I diabetes mellitus

Allograft rejection

mTOR signaling pathway

Renal cell carcinoma

T cell receptor signaling pathway

Long-term depression

Hypertrophic cardiomyopathy (HCM)

Axon guidance

NOD-like receptor signaling pathway

Fc epsilon RI signaling pathway

Calcium signaling pathway

VEGF signaling pathway

Apoptosis

Gap junction

Graft-versus-host disease

PPAR signaling pathway

Prion diseases

Vascular smooth muscle contraction

Asthma

Regulation of autophagy

Neurotrophin signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Adipocytokine signaling pathway

p53 signaling pathway

Glioma

Alzheimer's disease

Colorectal cancer

Small cell lung cancer

Primary immunodeficiency

Huntington's disease

Arachidonic acid metabolism

Ether lipid metabolism

Cell adhesion molecules (CAMs)

Fc gamma R-mediated phagocytosis

Viral myocarditis

Glycosphingolipid biosynthesis - lacto and neolacto series

Glycerophospholipid metabolism

Keratan sulfate biosynthesis

Renin-angiotensin system

Glycerolipid metabolism

Non-small cell lung cancer

Taste transduction

Vibrio cholerae infection

Glycosaminoglycan degradation

Linoleic acid metabolism

alpha-Linolenic acid metabolism

Lysosome

Adherens junction

Dorso-ventral axis formation

Endometrial cancer

Type II diabetes mellitus

Cell cycle

Oocyte meiosis

Progesterone-mediated oocyte maturation

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Amino sugar and nucleotide sugar metabolism

Chronic myeloid leukemia

Amyotrophic lateral sclerosis (ALS)

SNARE interactions in vesicular transport

O-Glycan biosynthesis

Leukocyte transendothelial migration

Purine metabolism

Pyrimidine metabolism

Maturity onset diabetes of the young

Glutathione metabolism

Histidine metabolism

N-Glycan biosynthesis

Starch and sucrose metabolism

Other glycan degradation

Biotin metabolism

Insulin signaling pathway

Proteasome

Glycosphingolipid biosynthesis - ganglio series

Glycosphingolipid biosynthesis - globo series

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Nitrogen metabolism

Pathogenic Escherichia coli infection

beta-Alanine metabolism

Acute myeloid leukemia

Arginine and proline metabolism

Tryptophan metabolism

Galactose metabolism

Riboflavin metabolism

Tyrosine metabolism

Folate biosynthesis

Human

E. coli

Human 293

Plasma

Baculovirus

HEK293

Mammalian

P.Pichia

Pancreas

Pituitary

Neomycin

Puromycin

None

1

2

Yes

In Stock