Gfi1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Purified recombinant protein of Human growth factor independent 1 transcription repressor (GFI1), transcript variant 3, full length, with N-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human growth factor independent 1 transcription repressor (GFI1), transcript variant 2, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Gfi1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
GFI1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
GFI1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
GFI1 CRISPRa kit - CRISPR gene activation of human growth factor independent 1 transcriptional repressor
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Gfi1 CRISPRa kit - CRISPR gene activation of mouse growth factor independent 1 transcription repressor
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of growth factor independent 1 transcription repressor (GFI1), transcript variant 3
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of growth factor independent 1 transcription repressor (GFI1), transcript variant 2
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
qSTAR qPCR primer pairs against Homo sapiens gene GFI1
qPCR primer pairs and template standards against Homo sapiens gene GFI1
| Application | Plasmid of exact quantity for transcript copy number calculation |
Transient overexpression of GFI1, transcript variant 2, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of GFI1, transcript variant 3, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of GFI1, transcript variant 1, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
