Recombinant protein of human SET domain containing (lysine methyltransferase) 8 (SETD8), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Products
KMT5A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
SETD8 mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)
| Applications | IHC, WB |
| Reactivities | Human |
KMT5A - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Kmt5a - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
KMT5A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SETD8 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)
| Applications | IHC, WB |
| Reactivities | Human |
Transient overexpression lysate of SET domain containing (lysine methyltransferase) 8 (SETD8)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Kmt5a CRISPRa kit - CRISPR gene activation of mouse lysine methyltransferase 5A
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
KMT5A CRISPRa kit - CRISPR gene activation of human lysine methyltransferase 5A
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
3' UTR clone of SET domain containing (lysine methyltransferase) 8 (SETD8) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qPCR primer pairs and template standards against Homo sapiens gene SETD8
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Mus musculus gene Kmt5a
Transient overexpression of KMT5A in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
qSTAR qPCR primer pairs against Homo sapiens gene KMT5A
