Products
Cytokeratin 14 human recombinant protein, 0.1 mg
| Expression Host | E. coli |
Cytokeratin 14 (KRT14) mouse monoclonal antibody, clone LL002, FITC
| Applications | FC, IF |
| Reactivities | Human |
Anti-Cytokeratin 14 [2G4], Mouse IgG1, kappa
| Applications | ELISA, IHC |
| Reactivities | Human |
Anti-Cytokeratin 14 [2G4], Rabbit IgG, kappa
| Applications | ELISA, IHC |
| Reactivities | Human |
| Modifications | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
Rabbit monoclonal to CK-14 (Cytokeratin-14)
| Applications | IHC, WB |
| Reactivities | Human, Mouse |
Cytokeratin 14 (KRT14) mouse monoclonal antibody, clone DE-SPK14, Purified
| Applications | IHC, WB |
| Reactivities | Human |
Cytokeratin 14 human recombinant protein, 0.25 mg
| Expression Host | E. coli |
Krt14 (Myc-DDK-tagged ORF) - Rat keratin 14 (Krt14), (10 ug)
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Krt14 (tGFP-tagged) - Mouse keratin 14 (Krt14)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Krt14 (mGFP-tagged) - Mouse keratin 14 (Krt14)
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
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- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (2) - both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- e.g. Dulbecco's PBS). (2)
-
pH 7.4).
For immunization purposes (2) - the solution can be further dialyzed against PBS (phosphate buffered saline (2)
