PAK6 CRISPRa kit - CRISPR gene activation of human p21 (RAC1) activated kinase 6
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Transient overexpression lysate of p21 protein (Cdc42/Rac)-activated kinase 6 (PAK6), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Pak6 CRISPRa kit - CRISPR gene activation of mouse p21 (RAC1) activated kinase 6
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of p21 protein (Cdc42/Rac)-activated kinase 6 (PAK6), transcript variant 2
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PAK6 - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
PAK6 - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
| Donor DNA | mBFP-Neo |
PAK6 - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
qSTAR qPCR primer pairs against Homo sapiens gene PAK6
qSTAR qPCR primer pairs against mus musculus gene Pak6
qPCR primer pairs and template standards against Homo sapiens gene PAK6
| Application | Plasmid of exact quantity for transcript copy number calculation |
Transient overexpression of BUB1B-PAK6, transcript variant 3, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of BUB1B-PAK6, transcript variant 2, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of PAK6, transcript variant 1, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Pak6 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Pak6 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
