Polr2e - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Polr2e (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (cDNA clone MGC:28579 IMAGE:4210827)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Recombinant protein of human polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
POLR2E mouse monoclonal antibody, clone OTI3C5 (formerly 3C5)
| Applications | IF, WB |
| Reactivities | Human, Mouse, Rat |
POLR2E mouse monoclonal antibody, clone OTI2A11 (formerly 2A11)
| Applications | IF, WB |
| Reactivities | Human, Mouse, Rat |
POLR2E CRISPRa kit - CRISPR gene activation of human RNA polymerase II subunit E
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
qPCR primer pairs and template standards against Homo sapiens gene POLR2E
| Application | Plasmid of exact quantity for transcript copy number calculation |
Purified recombinant protein of Mouse polymerase (RNA) II (DNA directed) polypeptide E (Polr2e), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
Polr2e (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene POLR2E
qSTAR qPCR primer pairs against Mus musculus gene Polr2e
Transient overexpression of POLR2E in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
POLR2E - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
