Puf60 CRISPRa kit - CRISPR gene activation of mouse poly-U binding splicing factor 60
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Transient overexpression lysate of poly-U binding splicing factor 60KDa (PUF60), transcript variant 2
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of poly-U binding splicing factor 60KDa (PUF60), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PUF60 MS Standard C13 and N15-labeled recombinant protein (NP_510965)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
PUF60 MS Standard C13 and N15-labeled recombinant protein (NP_055096)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
qPCR primer pairs and template standards against Homo sapiens gene PUF60
| Application | Plasmid of exact quantity for transcript copy number calculation |
Transient overexpression of PUF60, transcript variant 1, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of PUF60, transcript variant 2, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Puf60 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Puf60 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene PUF60
qSTAR qPCR primer pairs against Mus musculus gene Puf60
PUF60 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Puf60 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
