SEMA6D HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Products
SEMA6D CRISPRa kit - CRISPR gene activation of human semaphorin 6D
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 4
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 6
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Sema6d CRISPRa kit - CRISPR gene activation of mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qSTAR qPCR primer pairs against Homo sapiens gene SEMA6D
qPCR primer pairs and template standards against Mus musculus gene Sema6d
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against mus musculus gene Sema6d
Transient overexpression of SEMA6D, transcript variant 6, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of SEMA6D, transcript variant 4, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Sema6d - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
SEMA6D - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Sema6d (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Sema6d - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Sema6d - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
