Cyld CRISPRa kit - CRISPR gene activation of mouse CYLD lysine 63 deubiquitinase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
CYLD CRISPRa kit - CRISPR gene activation of human CYLD lysine 63 deubiquitinase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
CYLD - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
| Donor DNA | GFP-puro |
CYLD - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
CYLD - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
qSTAR qPCR primer pairs against mus musculus gene Cyld
CYLD - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
qSTAR qPCR primer pairs against Homo sapiens gene CYLD
qPCR primer pairs and template standards against Homo sapiens gene CYLD
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Mus musculus gene Cyld
| Application | Plasmid of exact quantity for transcript copy number calculation |
Purified recombinant protein of Mouse CYLD lysine 63 deubiquitinase (Cyld), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
Cyld (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Transient overexpression of CYLD, transcript variant 2, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of CYLD, transcript variant 3, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Cyld - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
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Species
Applications
Antibody Hosts
Expression Hosts
Lentivirus
Vectors
- pCMV6-Entry (13)
- pLenti-C-Myc-DDK-P2A-Puro (8)
- pLenti-C-mGFP-P2A-Puro (8)
- pCMV6-AC-GFP (7)
- pGFP-C-shLenti (3)
- pRS (3)
- EF1a-GFP-P2A-Puro (2)
- pLenti-C-Myc-DDK (2)
- pLenti-C-mGFP (2)
- GFP-puro (1)
- Luciferase-Puro (1)
- PCMV6-Kan/Neo (1)
- RFP-BSD (1)
- mBFP-Neo (1)
- pCMV6-AC (1)
- pCMV6-XL5 (1)
- pGFP-V-RS (1)
- pRFP-C-RS (1)
