Desmin Rabbit polyclonal Antibody
| Applications | ELISA, WB |
| Reactivities | Mouse |
| Modifications | Unmodified |
Products
Desmin (DES) mouse monoclonal antibody, clone D33, Purified
| Applications | IF, IHC, WB |
| Reactivities | Chicken, Hamster, Human, Rat |
Desmin (DES) mouse monoclonal antibody, clone D33, Supernatant
| Applications | IF, IHC, WB |
| Reactivities | Human |
Desmin Mouse Monoclonal Antibody
| Applications | IHC |
| Reactivities | Human |
Desmin (DES) rabbit polyclonal antibody, Purified
| Applications | IHC |
| Reactivities | Human |
Desmin (DES) rabbit polyclonal antibody, Purified
| Applications | IHC |
| Reactivities | Human |
Desmin chicken protein, 0.1 mg
| Protein Source | Stomach |
Desmin chicken protein, 0.25 mg
| Protein Source | Stomach |
Des (tGFP-tagged) - Mouse desmin (Des)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Des (mGFP-tagged) - Mouse desmin (Des)
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Des (Myc-DDK-tagged) - Mouse desmin (Des)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Des (untagged) - Mouse desmin (Des), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
DES (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
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Species
Reactivities
Antibody Hosts
Expression Hosts
Lentivirus
Vectors
Reporters
Conjugates
Modifications
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Assay Types
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Families
- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization. (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) - e.g. Dulbecco's PBS). (2)
- pH 7.4). For immunization purposes (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
