PHGDH mouse monoclonal antibody, clone OTI4A1 (formerly 4A1)
| Applications | WB |
| Reactivities | Human, Mouse, Rat |
Products
PHGDH - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Lenti ORF clone of Human phosphoglycerate dehydrogenase (PHGDH), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
PHGDH CRISPRa kit - CRISPR gene activation of human phosphoglycerate dehydrogenase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Phgdh CRISPRa kit - CRISPR gene activation of mouse 3-phosphoglycerate dehydrogenase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
PHGDH - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
Phgdh - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
qPCR primer pairs and template standards against Homo sapiens gene PHGDH
| Application | Plasmid of exact quantity for transcript copy number calculation |
Purified recombinant protein of Mouse 3-phosphoglycerate dehydrogenase (Phgdh), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
Phgdh (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene PHGDH
qSTAR qPCR primer pairs against Mus musculus gene Phgdh
Transient overexpression of PHGDH in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Phgdh - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Phgdh - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
