DES (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Products
Des (untagged ORF) - Rat desmin (Des), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
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- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization. (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) - e.g. Dulbecco's PBS). (2)
- pH 7.4). For immunization purposes (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
