TXN MS Standard C13 and N15-labeled recombinant protein (NP_003320)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
Products
TXN (untagged) - Homo sapiens thioredoxin (TXN), transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
3' UTR clone of thioredoxin (TXN) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
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- Druggable Genome (28)
- 0.1% (w/v) insulin (2)
- 1mM DTT (2)
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2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (2) - 63mM sodium phosphate (2)
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Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (2) - WB (2)
