Cd47 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
CD47 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
CD47 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Purified recombinant protein of Homo sapiens CD47 molecule (CD47), residues 19-141aa, with C-terminal Fc tag, expressed in HEK293 cells.
| Tag | C-Fc |
| Expression Host | HEK293T |
Cd47 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Transient overexpression lysate of CD47 molecule (CD47), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Purified recombinant protein of Human CD47 molecule (CD47), transcript variant 1, residues 19-141aa, with C-terminal DDK tag, expressed in sf9, 20ug
| Tag | C-DDK |
| Expression Host | Sf9 |
Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK |
Cd47 CRISPRa kit - CRISPR gene activation of mouse CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
CD47 CRISPRa kit - CRISPR gene activation of human CD47 molecule
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qSTAR qPCR primer pairs against Homo sapiens gene CD47
qSTAR qPCR primer pairs against Mus musculus gene Cd47
CD47 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
CD47 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
qPCR primer pairs and template standards against Homo sapiens gene CD47
| Application | Plasmid of exact quantity for transcript copy number calculation |
