ACOT1 (Myc-DDK-tagged)-Human acyl-CoA thioesterase 1 (ACOT1)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Recombinant protein of human acyl-CoA thioesterase 1 (ACOT1), 20 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
ACOT1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
ACOT1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Recombinant protein of human acyl-CoA thioesterase 1 (ACOT1), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human acyl-CoA thioesterase 1 (ACOT1), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Purified recombinant protein of Human acyl-CoA thioesterase 1 (ACOT1), full length, with N-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Transient overexpression lysate of acyl-CoA thioesterase 1 (ACOT1)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
ACOT1 CRISPRa kit - CRISPR gene activation of human acyl-CoA thioesterase 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene ACOT1
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene ACOT1
Transient overexpression of ACOT1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
ACOT1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
ACOT1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
ACOT1 (tGFP-tagged) - Human acyl-CoA thioesterase 1 (ACOT1)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
