DES CRISPRa kit - CRISPR gene activation of human desmin
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
qSTAR qPCR primer pairs against Homo sapiens gene DES
qPCR primer pairs and template standards against Homo sapiens gene DES
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene DES
| Application | Plasmid of exact quantity for transcript copy number calculation |
DES (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
活动过滤器
优化搜索结果
Expression Hosts
Families
- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) - e.g. Dulbecco's PBS). (2)
- pH 7.4). For immunization purposes (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
