DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Products
DOT1L - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Transient overexpression lysate of DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
DOT1L - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
DOT1L (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Purified recombinant protein of Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L), Met1-Asp434, with N-terminal His tag, expressed in E.coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
DOT1L CRISPRa kit - CRISPR gene activation of human DOT1 like histone lysine methyltransferase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qSTAR qPCR primer pairs against Homo sapiens gene DOT1L
Transient overexpression of DOT1L in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
DOT1L - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
DOT1L (tGFP-tagged) - Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti-ORF clone of DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP |
- LentiORF®
Lenti-ORF clone of DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti-ORF clone of DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK |
- LentiORF®
Lenti-ORF clone of DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
