HES1 (Myc-DDK-tagged)-Human hairy and enhancer of split 1, (Drosophila) (HES1)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Recombinant protein of human hairy and enhancer of split 1, (Drosophila) (HES1), 20 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
HES1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
HES1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
HES1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Purified recombinant protein of Human hairy and enhancer of split 1, (Drosophila) (HES1), with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
HES1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Recombinant protein of human hairy and enhancer of split 1, (Drosophila) (HES1), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human hairy and enhancer of split 1, (Drosophila) (HES1), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of hairy and enhancer of split 1, (Drosophila) (HES1)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
HES1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
HES1 CRISPRa kit - CRISPR gene activation of human hes family bHLH transcription factor 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qSTAR qPCR primer pairs against Homo sapiens gene HES1
qPCR primer pairs and template standards against Homo sapiens gene HES1
| Application | Plasmid of exact quantity for transcript copy number calculation |
Transient overexpression of HES1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
