SETD8 (Myc-DDK-tagged)-Human SET domain containing (lysine methyltransferase) 8 (SETD8)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Recombinant protein of human SET domain containing (lysine methyltransferase) 8 (SETD8), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
KMT5A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
KMT5A - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
KMT5A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Transient overexpression lysate of SET domain containing (lysine methyltransferase) 8 (SETD8)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
KMT5A CRISPRa kit - CRISPR gene activation of human lysine methyltransferase 5A
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
3' UTR clone of SET domain containing (lysine methyltransferase) 8 (SETD8) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qPCR primer pairs and template standards against Homo sapiens gene SETD8
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene KMT5A
Transient overexpression of KMT5A in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
KMT5A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
SETD8 (tGFP-tagged) - Human SET domain containing (lysine methyltransferase) 8 (SETD8)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Human SET domain containing (lysine methyltransferase) 8 (SETD8), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Human SET domain containing (lysine methyltransferase) 8 (SETD8), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK |
- LentiORF®
