NODAL (Myc-DDK-tagged)-Human nodal homolog (mouse) (NODAL)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
NODAL - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
NODAL (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Purified recombinant protein of Human nodal homolog (mouse) (NODAL), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Transient overexpression lysate of nodal homolog (mouse) (NODAL)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
NODAL - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
NODAL - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
NODAL CRISPRa kit - CRISPR gene activation of human nodal growth differentiation factor
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qSTAR qPCR primer pairs against Homo sapiens gene NODAL
NODAL - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
qPCR primer pairs and template standards against Homo sapiens gene NODAL
| Application | Plasmid of exact quantity for transcript copy number calculation |
Transient overexpression of NODAL in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
NODAL - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
NODAL (tGFP-tagged) - Human nodal homolog (mouse) (NODAL)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Human nodal homolog (mouse) (NODAL), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK |
- LentiORF®
